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RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells.

Publication ,  Journal Article
Penalva, LOF; Burdick, MD; Lin, SM; Sutterluety, H; Keene, JD
Published in: Mol Cancer
September 7, 2004

BACKGROUND: Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes. RESULTS: In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. Moreover, using this approach we identify a set of endothelial genes that respond to the presence of co-cultured breast tumor cells. CONCLUSION: RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors.

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Published In

Mol Cancer

DOI

EISSN

1476-4598

Publication Date

September 7, 2004

Volume

3

Start / End Page

24

Location

England

Related Subject Headings

  • RNA, Messenger
  • Poly(A)-Binding Proteins
  • Oncology & Carcinogenesis
  • Mice
  • Mammary Neoplasms, Experimental
  • Humans
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Epitopes
  • Endothelial Cells
 

Citation

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Penalva, L. O. F., Burdick, M. D., Lin, S. M., Sutterluety, H., & Keene, J. D. (2004). RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells. Mol Cancer, 3, 24. https://doi.org/10.1186/1476-4598-3-24
Penalva, Luiz O. F., Michael D. Burdick, Simon M. Lin, Hedwig Sutterluety, and Jack D. Keene. “RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells.Mol Cancer 3 (September 7, 2004): 24. https://doi.org/10.1186/1476-4598-3-24.
Penalva LOF, Burdick MD, Lin SM, Sutterluety H, Keene JD. RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells. Mol Cancer. 2004 Sep 7;3:24.
Penalva, Luiz O. F., et al. “RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells.Mol Cancer, vol. 3, Sept. 2004, p. 24. Pubmed, doi:10.1186/1476-4598-3-24.
Penalva LOF, Burdick MD, Lin SM, Sutterluety H, Keene JD. RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells. Mol Cancer. 2004 Sep 7;3:24.
Journal cover image

Published In

Mol Cancer

DOI

EISSN

1476-4598

Publication Date

September 7, 2004

Volume

3

Start / End Page

24

Location

England

Related Subject Headings

  • RNA, Messenger
  • Poly(A)-Binding Proteins
  • Oncology & Carcinogenesis
  • Mice
  • Mammary Neoplasms, Experimental
  • Humans
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Epitopes
  • Endothelial Cells