RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells.

Published online

Journal Article

BACKGROUND: Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes. RESULTS: In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. Moreover, using this approach we identify a set of endothelial genes that respond to the presence of co-cultured breast tumor cells. CONCLUSION: RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors.

Full Text

Duke Authors

Cited Authors

  • Penalva, LOF; Burdick, MD; Lin, SM; Sutterluety, H; Keene, JD

Published Date

  • September 7, 2004

Published In

Volume / Issue

  • 3 /

Start / End Page

  • 24 -

PubMed ID

  • 15353001

Pubmed Central ID

  • 15353001

Electronic International Standard Serial Number (EISSN)

  • 1476-4598

Digital Object Identifier (DOI)

  • 10.1186/1476-4598-3-24

Language

  • eng

Conference Location

  • England