Jack Donald Keene
James B. Duke Distinguished Professor of Molecular Genetics and Microbiology
The Keene Laboratory has a long-term interest in the structure and function of viral and mammalian genomes. Having determined the first genomic sequences for rabies, Ebola, Marburg and vesicular stomatitis virus, and discerned the origins of defective interfering viruses, interests shifted to the cloning of six human genes involved in autoimmune reactivity. This resulted in the identification of numerous autoimmune RRM-type RNA-binding proteins the discovery of the RRM, and the RNA targets to which they bind. The current interests of the lab surround the functions of the human RRM-ELAV/Hu proteins that are bound to a subset of cellular mRNAs involved in growth regulation neuronal plasticiyt and cancer. The laboratory demonstrated that ELAV/Hu proteins bind and regulate the expression of early response gene transcripts such as those encoding the protooncogene and cytokine proteins.
In addition, it was shown that while stabilizing these mRNAs and/or activating their translation, the ELAV/Hu proteins participate in cellular , differentiation and carcinogenesis. More recently, the laboratory has examined dozens of RNA-binding proteins in order to identify large numbers of structurally
and/or functionally related mRNAs that cluster in vivo based upon their binding to these proteins. This has been termed ribonomics because it involves parallel analysis of mRNA subsets en masse based upon their presence in messenger ribonucleoprotein complexes. This new approach to functional genomics is being applied to virus-infected cells, tumors and cells treated with various agents. Ribonomics has led to the identification of mRNA clusters that are posttranscriptionally regulated, and represent the organizational state of genetic information between the genome and the proteome. Dr. Keen has propsed the existence of post-transcriptional operons based upon the association of structurally and functionally-linked mRNAs in vivo.
In addition, it was shown that while stabilizing these mRNAs and/or activating their translation, the ELAV/Hu proteins participate in cellular , differentiation and carcinogenesis. More recently, the laboratory has examined dozens of RNA-binding proteins in order to identify large numbers of structurally
and/or functionally related mRNAs that cluster in vivo based upon their binding to these proteins. This has been termed ribonomics because it involves parallel analysis of mRNA subsets en masse based upon their presence in messenger ribonucleoprotein complexes. This new approach to functional genomics is being applied to virus-infected cells, tumors and cells treated with various agents. Ribonomics has led to the identification of mRNA clusters that are posttranscriptionally regulated, and represent the organizational state of genetic information between the genome and the proteome. Dr. Keen has propsed the existence of post-transcriptional operons based upon the association of structurally and functionally-linked mRNAs in vivo.
Current Appointments & Affiliations
- James B. Duke Distinguished Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 1997
- Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 1989
- Associate Professor in Medicine, Medicine, Rheumatology and Immunology, Medicine 2020
- Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 1979
Contact Information
- 207 Research Drive, 414 Jones Box 3020, Durham, NC 27717
- Duke Box 3020, Durham, NC 27710
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keene001@mc.duke.edu
(919) 684-5138
- Background
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Education, Training, & Certifications
- Ph.D., University of Washington 1974
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Duke Appointment History
- Associate Professor in Medicine, Medicine, Rheumatology and Immunology, Medicine 2006 - 2020
- Associate Research Professor in Medicine, Medicine, Rheumatology and Immunology, Medicine 1999 - 2006
- Director, Center for RNA Biology, Molecular Genetics and Microbiology, Basic Science Departments 2002 - 2006
- Director of Basic Science Research, Comprehensive Cancer Center, Duke Cancer Institute, Institutes and Centers 1995 - 2003
- Chair, Department of Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 1994 - 2002
- Associate Research Professor in Medicine, Medicine, Clinical Science Departments 1994 - 1999
- Recognition
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Awards & Honors
- Honorary Member. The LARP (La-And-Related-Protein) Society. 2014
- AAAS Fellow. The American Association for the Advancement of Science. 2010
- Elected Member. The Henry Kunkel Honorary Society. 2000
- Fellow of the American Academy of Microbiology. American Society of Microbiology. 1991
- Pew Scholar in the Biomedical Sciences. The Pew Charitable Trusts. 1985
- Research
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Selected Grants
- Genetic and Genomics Training Grant awarded by National Institutes of Health 2020 - 2025
- Distal mRNA localization and translation in neural stem cells of the developing brain awarded by National Institutes of Health 2018 - 2023
- Bioinformatics and Computational Biology Training Program awarded by National Institutes of Health 2005 - 2021
- Transfusion Medicine and Hematology awarded by National Institutes of Health 1975 - 2021
- Genetics Training Grant awarded by National Institutes of Health 1979 - 2020
- Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
- Interferon stimulated genes that regulate viral infection by binding to modified RNAs awarded by National Institutes of Health 2019 - 2020
- Post-transcriptional RNA regulation in mammalian neural stem cells awarded by National Institutes of Health 2017 - 2020
- Targeting Translation Control in Malignant Glioma awarded by National Institutes of Health 2007 - 2018
- Post-transcriptional coordination by HuR and microRNAs during tumorigenesis awarded by National Institutes of Health 2014 - 2017
- RNA Dynamics of Transient Macromolecular Complexes in Cancer Cells awarded by National Institutes of Health 2011 - 2017
- Cancer Biology Training Grant awarded by National Cancer Institute 1993 - 2016
- Clinical Oncology Research Career Development Program awarded by National Institutes of Health 2009 - 2015
- Regulation of Germ Cell Pluripotency Through The RNA-Binding Protein, DND1 awarded by National Institutes of Health 2010 - 2014
- RNA Operons In Coordinated Gene Expression and Horizontal Transfer awarded by National Science Foundation 2009 - 2012
- Mucin Gene Regulation by Elastase and Oxidants awarded by National Institutes of Health 2005 - 2010
- Development And Persistence Of Immunity In SCID Chimeras awarded by National Institutes of Health 1998 - 2010
- Posttranscriptional regulation of the Wnt pathway by HuR and microRNAs awarded by National Institutes of Health 2007 - 2009
- Endothelial Cell Molecular Alterations in Cancer awarded by National Institutes of Health 2002 - 2008
- Posttranscriptional Gene Regulation in T Cell Activation awarded by National Institutes of Health 2000 - 2007
- Stem Cell Mechanism in the Murine Germline awarded by National Institutes of Health 2002 - 2006
- Function of Mammalian ELAV RNA-Binding Proteins awarded by National Institutes of Health 1999 - 2006
- Regulation of Mucin Gene Expression by Elastase awarded by National Institutes of Health 2000 - 2005
- Cancer Center Core Support Grant awarded by National Institutes of Health 1976 - 1998
- Comprehensive Cancer Center Core Support Grant awarded by National Institutes of Health 1976 - 1998
- Comprehensive Cancer Center Core Support Grant awarded by National Institutes of Health 1976 - 1998
- Determinants Of Specificity In Protein-Rna Interactions awarded by National Institutes of Health 1993 - 1998
- Peptide Immunogens For Mucosal & Systemic Hiv Vaccines Nat awarded by National Institutes of Health 1993 - 1997
- Molecular Biology Of The Ro-Rnp Autoantigen awarded by National Institutes of Health 1993 - 1994
- Replicative Mechanisms Of The Negative Strand Viruses awarded by National Institutes of Health 1992 - 1993
- Replicative Mechanisms Of The Negative Strand Viruses awarded by National Institutes of Health 1991 - 1993
- Replicative Mechanisms Of The Negative Strant Viruses awarded by National Institutes of Health 1990 - 1993
- Replicative Mechnaisms Of The Negative Strand Viruses awarded by National Institutes of Health 1986 - 1988
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External Relationships
- medical and biological laboratories
- Publications & Artistic Works
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Selected Publications
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Academic Articles
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Ruthig, Victor A., Tetsuhiro Yokonishi, Matthew B. Friedersdorf, Sofia Batchvarova, Josiah Hardy, Jason A. Garness, Jack D. Keene, and Blanche Capel. “A transgenic DND1GFP fusion allele reports in vivo expression and RNA-binding targets in undifferentiated mouse germ cells†.” Biol Reprod 104, no. 4 (April 1, 2021): 861–74. https://doi.org/10.1093/biolre/ioaa233.Full Text Link to Item
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Ruthig, Victor A., Matthew B. Friedersdorf, Jason A. Garness, Steve C. Munger, Corey Bunce, Jack D. Keene, and Blanche Capel. “The RNA-binding protein DND1 acts sequentially as a negative regulator of pluripotency and a positive regulator of epigenetic modifiers required for germ cell reprogramming.” Development 146, no. 19 (July 25, 2019). https://doi.org/10.1242/dev.175950.Full Text Open Access Copy Link to Item
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Bisogno, Laura S., Matthew B. Friedersdorf, and Jack D. Keene. “Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion.” Iscience 4 (June 29, 2018): 97–108. https://doi.org/10.1016/j.isci.2018.05.011.Full Text Open Access Copy Link to Item
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Bisogno, Laura Simone, and Jack Donald Keene. “RNA regulons in cancer and inflammation.” Curr Opin Genet Dev 48 (February 2018): 97–103. https://doi.org/10.1016/j.gde.2017.11.004.Full Text Link to Item
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Wang, Sihyung, Youngmi Jung, Jeongeun Hyun, Matthew Friedersdorf, Seh-Hoon Oh, Jieun Kim, Richard T. Premont, Jack D. Keene, and Anna Mae Diehl. “RNA Binding Proteins Control Transdifferentiation of Hepatic Stellate Cells into Myofibroblasts.” Cell Physiol Biochem 48, no. 3 (2018): 1215–29. https://doi.org/10.1159/000491987.Full Text Link to Item
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Bisogno, Laura S., and Jack D. Keene. “Analysis of post-transcriptional regulation during cancer progression using a donor-derived isogenic model of tumorigenesis.” Methods 126 (August 15, 2017): 193–200. https://doi.org/10.1016/j.ymeth.2017.05.012.Full Text Link to Item
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Nicholson, Cindo O., Matthew B. Friedersdorf, Laura S. Bisogno, and Jack D. Keene. “DO-RIP-seq to quantify RNA binding sites transcriptome-wide.” Methods 118–119 (April 15, 2017): 16–23. https://doi.org/10.1016/j.ymeth.2016.11.004.Full Text Link to Item
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Nicholson, Cindo O., Matthew Friedersdorf, and Jack D. Keene. “Quantifying RNA binding sites transcriptome-wide using DO-RIP-seq.” Rna 23, no. 1 (January 2017): 32–46. https://doi.org/10.1261/rna.058115.116.Full Text Link to Item
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Smola, Matthew J., Thomas W. Christy, Kaoru Inoue, Cindo O. Nicholson, Matthew Friedersdorf, Jack D. Keene, David M. Lee, J Mauro Calabrese, and Kevin M. Weeks. “SHAPE reveals transcript-wide interactions, complex structural domains, and protein interactions across the Xist lncRNA in living cells.” Proc Natl Acad Sci U S A 113, no. 37 (September 13, 2016): 10322–27. https://doi.org/10.1073/pnas.1600008113.Full Text Link to Item
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Blackinton, Jeff G., and Jack D. Keene. “Functional coordination and HuR-mediated regulation of mRNA stability during T cell activation.” Nucleic Acids Res 44, no. 1 (January 8, 2016): 426–36. https://doi.org/10.1093/nar/gkv1066.Full Text Open Access Copy Link to Item
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Moon, Stephanie L., Jeffrey G. Blackinton, John R. Anderson, Mary K. Dozier, Benjamin J. T. Dodd, Jack D. Keene, Carol J. Wilusz, Shelton S. Bradrick, and Jeffrey Wilusz. “XRN1 stalling in the 5' UTR of Hepatitis C virus and Bovine Viral Diarrhea virus is associated with dysregulated host mRNA stability.” Plos Pathog 11, no. 3 (March 2015): e1004708. https://doi.org/10.1371/journal.ppat.1004708.Full Text Link to Item
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Jiang, Huifeng, Lin Xu, Zhe Wang, Jack Keene, and Zhenglong Gu. “Coordinating expression of RNA binding proteins with their mRNA targets.” Sci Rep 4 (November 24, 2014): 7175. https://doi.org/10.1038/srep07175.Full Text Link to Item
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Blackinton, Jeff G., and Jack D. Keene. “Post-transcriptional RNA regulons affecting cell cycle and proliferation.” Semin Cell Dev Biol 34 (October 2014): 44–54. https://doi.org/10.1016/j.semcdb.2014.05.014.Full Text Link to Item
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Payne, Tamika L., Jeff Blackinton, Alyse Frisbee, Joy Pickeral, Sheetal Sawant, Nathan A. Vandergrift, Stephanie A. Freel, Guido Ferrari, Jack D. Keene, and Georgia D. Tomaras. “Transcriptional and posttranscriptional regulation of cytokine gene expression in HIV-1 antigen-specific CD8+ T cells that mediate virus inhibition.” J Virol 88, no. 17 (September 1, 2014): 9514–28. https://doi.org/10.1128/JVI.00802-14.Full Text Link to Item
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Keene, Jack D. “The globalization of messenger RNA regulation.” Natl Sci Rev 1, no. 2 (June 2014): 184–86. https://doi.org/10.1093/nsr/nwu004.Full Text Link to Item
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Friedersdorf, Matthew B., and Jack D. Keene. “Advancing the functional utility of PAR-CLIP by quantifying background binding to mRNAs and lncRNAs.” Genome Biol 15, no. 1 (January 7, 2014): R2. https://doi.org/10.1186/gb-2014-15-1-r2.Full Text Link to Item
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Simone, Laura E., and Jack D. Keene. “Mechanisms coordinating ELAV/Hu mRNA regulons.” Curr Opin Genet Dev 23, no. 1 (February 2013): 35–43. https://doi.org/10.1016/j.gde.2012.12.006.Full Text Link to Item
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Mansfield, Kyle D., and Jack D. Keene. “Neuron-specific ELAV/Hu proteins suppress HuR mRNA during neuronal differentiation by alternative polyadenylation.” Nucleic Acids Res 40, no. 6 (March 2012): 2734–46. https://doi.org/10.1093/nar/gkr1114.Full Text Link to Item
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Zarate, C. A., I. D. Henter, and H. K. Manji. “Preface.” Current Topics in Behavioral Neurosciences 5, no. 1 (December 1, 2011).
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Corcoran, David L., Stoyan Georgiev, Neelanjan Mukherjee, Eva Gottwein, Rebecca L. Skalsky, Jack D. Keene, and Uwe Ohler. “PARalyzer: definition of RNA binding sites from PAR-CLIP short-read sequence data.” Genome Biol 12, no. 8 (August 18, 2011): R79. https://doi.org/10.1186/gb-2011-12-8-r79.Full Text Link to Item
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Mukherjee, Neelanjan, David L. Corcoran, Jeffrey D. Nusbaum, David W. Reid, Stoyan Georgiev, Markus Hafner, Manuel Ascano, Thomas Tuschl, Uwe Ohler, and Jack D. Keene. “Integrative regulatory mapping indicates that the RNA-binding protein HuR couples pre-mRNA processing and mRNA stability.” Mol Cell 43, no. 3 (August 5, 2011): 327–39. https://doi.org/10.1016/j.molcel.2011.06.007.Full Text Open Access Copy Link to Item
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Mazan-Mamczarz, Krystyna, Patrick R. Hagner, Yongqing Zhang, Bojie Dai, Elin Lehrmann, Kevin G. Becker, Jack D. Keene, Myriam Gorospe, Zhenqui Liu, and Ronald B. Gartenhaus. “ATM regulates a DNA damage response posttranscriptional RNA operon in lymphocytes.” Blood 117, no. 8 (February 24, 2011): 2441–50. https://doi.org/10.1182/blood-2010-09-310987.Full Text Link to Item
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Neplioueva, Valentina, Elena Y. Dobrikova, Neelanjan Mukherjee, Jack D. Keene, and Matthias Gromeier. “Tissue type-specific expression of the dsRNA-binding protein 76 and genome-wide elucidation of its target mRNAs.” Plos One 5, no. 7 (July 23, 2010): e11710. https://doi.org/10.1371/journal.pone.0011710.Full Text Open Access Copy Link to Item
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Keene, Jack D. “The global dynamics of RNA stability orchestrates responses to cellular activation.” Bmc Biol 8 (July 8, 2010): 95. https://doi.org/10.1186/1741-7007-8-95.Full Text Link to Item
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Keene, Jack D. “Minireview: global regulation and dynamics of ribonucleic Acid.” Endocrinology 151, no. 4 (April 2010): 1391–97. https://doi.org/10.1210/en.2009-1250.Full Text Link to Item
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Morris, Adam R., Neelanjan Mukherjee, and Jack D. Keene. “Systematic analysis of posttranscriptional gene expression.” Wiley Interdiscip Rev Syst Biol Med 2, no. 2 (March 2010): 162–80. https://doi.org/10.1002/wsbm.54.Full Text Link to Item
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Papadaki, Olympia, Stavros Milatos, Sofia Grammenoudi, Neelanjan Mukherjee, Jack D. Keene, and Dimitris L. Kontoyiannis. “Control of thymic T cell maturation, deletion and egress by the RNA-binding protein HuR.” J Immunol 182, no. 11 (June 1, 2009): 6779–88. https://doi.org/10.4049/jimmunol.0900377.Full Text Link to Item
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Graner, Michael W., Oscar Alzate, Angelika M. Dechkovskaia, Jack D. Keene, John H. Sampson, Duane A. Mitchell, and Darell D. Bigner. “Proteomic and immunologic analyses of brain tumor exosomes.” Faseb J 23, no. 5 (May 2009): 1541–57. https://doi.org/10.1096/fj.08-122184.Full Text Link to Item
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Mansfield, Kyle D., and Jack D. Keene. “The ribonome: a dominant force in co-ordinating gene expression.” Biol Cell 101, no. 3 (March 2009): 169–81. https://doi.org/10.1042/BC20080055.Full Text Link to Item
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Mukherjee, Neelanjan, Patrick J. Lager, Matthew B. Friedersdorf, Marshall A. Thompson, and Jack D. Keene. “Coordinated posttranscriptional mRNA population dynamics during T-cell activation.” Mol Syst Biol 5 (2009): 288. https://doi.org/10.1038/msb.2009.44.Full Text Link to Item
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Tiruchinapalli, Dhanrajan M., Marc G. Caron, and Jack D. Keene. “Activity-dependent expression of ELAV/Hu RBPs and neuronal mRNAs in seizure and cocaine brain.” J Neurochem 107, no. 6 (December 2008): 1529–43. https://doi.org/10.1111/j.1471-4159.2008.05718.x.Full Text Link to Item
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Mazan-Mamczarz, K., P. R. Hagner, S. Corl, S. Srikantan, W. H. Wood, K. G. Becker, M. Gorospe, J. D. Keene, A. S. Levenson, and R. B. Gartenhaus. “Post-transcriptional gene regulation by HuR promotes a more tumorigenic phenotype.” Oncogene 27, no. 47 (October 16, 2008): 6151–63. https://doi.org/10.1038/onc.2008.215.Full Text Link to Item
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Tiruchinapalli, Dhanrajan M., Michael D. Ehlers, and Jack D. Keene. “Activity-dependent expression of RNA binding protein HuD and its association with mRNAs in neurons.” Rna Biol 5, no. 3 (July 2008): 157–68. https://doi.org/10.4161/rna.5.3.6782.Full Text Link to Item
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Morris, Adam R., Neelanjan Mukherjee, and Jack D. Keene. “Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets.” Mol Cell Biol 28, no. 12 (June 2008): 4093–4103. https://doi.org/10.1128/MCB.00155-08.Full Text Link to Item
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Pyhtila, Brook, Tianli Zheng, Patrick J. Lager, Jack D. Keene, Mary C. Reedy, and Christopher V. Nicchitta. “Signal sequence- and translation-independent mRNA localization to the endoplasmic reticulum.” Rna 14, no. 3 (March 2008): 445–53. https://doi.org/10.1261/rna.721108.Full Text Link to Item
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Mazroui, Rachid, Sergio Di Marco, Eveline Clair, Christopher von Roretz, Scott A. Tenenbaum, Jack D. Keene, Maya Saleh, and Imed-Eddine Gallouzi. “Caspase-mediated cleavage of HuR in the cytoplasm contributes to pp32/PHAP-I regulation of apoptosis.” J Cell Biol 180, no. 1 (January 14, 2008): 113–27. https://doi.org/10.1083/jcb.200709030.Full Text Link to Item
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Beach, Dale L., and Jack D. Keene. “Ribotrap : targeted purification of RNA-specific RNPs from cell lysates through immunoaffinity precipitation to identify regulatory proteins and RNAs.” Methods Mol Biol 419 (2008): 69–91. https://doi.org/10.1007/978-1-59745-033-1_5.Full Text Link to Item
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Konopka, Jennifer L., Luiz O. Penalva, Joseph M. Thompson, Laura J. White, Clayton W. Beard, Jack D. Keene, and Robert E. Johnston. “A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.” Plos Pathog 3, no. 12 (December 2007): e199. https://doi.org/10.1371/journal.ppat.0030199.Full Text Link to Item
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Keene, Jack D. “RNA regulons: coordination of post-transcriptional events.” Nat Rev Genet 8, no. 7 (July 2007): 533–43. https://doi.org/10.1038/nrg2111.Full Text Link to Item
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Keene, J. D. “Biological clocks and the coordination theory of RNA operons and regulons.” Cold Spring Harb Symp Quant Biol 72 (2007): 157–65. https://doi.org/10.1101/sqb.2007.72.013.Full Text Link to Item
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Konopka, J. L., L. O. Penalva, J. M. Thompson, L. J. White, C. W. Beard, J. D. Keene, and R. E. Johnston. “A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.” Plos Pathogens 3, no. 12 (2007): 2038–51. https://doi.org/10.1371/journal.ppat.0030199.Full Text
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Keene, J. D., J. M. Komisarow, and M. B. Friedersdorf. “Erratum: RIP-Chip: The isolation and identification of mRNAs, microRNAS and protein components of ribonucleoprotein complexes from cell extracts (Nature Protocols (2006) vol. 1 (302-307) 10.1038/nprot.2006.47).” Nature Protocols 1, no. 1 (June 1, 2006).
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Keene, Jack D., Jordan M. Komisarow, and Matthew B. Friedersdorf. “RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts.” Nat Protoc 1, no. 1 (2006): 302–7. https://doi.org/10.1038/nprot.2006.47.Full Text Link to Item
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Stephens, Samuel B., Rebecca D. Dodd, Joseph W. Brewer, Patrick J. Lager, Jack D. Keene, and Christopher V. Nicchitta. “Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation.” Mol Biol Cell 16, no. 12 (December 2005): 5819–31. https://doi.org/10.1091/mbc.e05-07-0685.Full Text Link to Item
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Keene, J. D., and P. J. Lager. “Erratum: Posttranscriptional operons and regulons coordinating gene expression (Chromosome Research (2005) 13:3 (327-337)).” Chromosome Research 13, no. 4 (June 1, 2005): 424.
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Keene, Jack D., and Patrick J. Lager. “Post-transcriptional operons and regulons co-ordinating gene expression.” Chromosome Res 13, no. 3 (2005): 327–37. https://doi.org/10.1007/s10577-005-0848-1.Full Text Link to Item
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Penalva, Luiz O. F., and Jack D. Keene. “Biotinylated tags for recovery and characterization of ribonucleoprotein complexes.” Biotechniques 37, no. 4 (October 2004): 604–10. https://doi.org/10.2144/04374ST05.Full Text Link to Item
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Penalva, Luiz O. F., Michael D. Burdick, Simon M. Lin, Hedwig Sutterluety, and Jack D. Keene. “RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells.” Mol Cancer 3 (September 7, 2004): 24. https://doi.org/10.1186/1476-4598-3-24.Full Text Link to Item
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Keene, J. D. “Microbiology and Molecular Biology Reviews: Note from the editor in chief.” Microbiology and Molecular Biology Reviews 68, no. 2 (June 1, 2004).
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Mesarovic, M. D., S. N. Sreenath, and J. D. Keene. “Search for organising principles: understanding in systems biology.” Syst Biol (Stevenage) 1, no. 1 (June 2004): 19–27. https://doi.org/10.1049/sb:20045010.Full Text Link to Item
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Kenan, Daniel J., and Jack D. Keene. “La gets its wings.” Nat Struct Mol Biol 11, no. 4 (April 2004): 303–5. https://doi.org/10.1038/nsmb0404-303.Full Text Link to Item
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Hallett, M., L. H. Phillips, D. L. Schomer, and J. M. Massey. “Preface.” Supplements to Clinical Neurophysiology 57, no. C (January 1, 2004): 5. https://doi.org/10.1016/S1567-424X(09)70335-6.Full Text
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Penalva, Luiz O. F., Scott A. Tenenbaum, and Jack D. Keene. “Gene expression analysis of messenger RNP complexes.” Methods Mol Biol 257 (2004): 125–34. https://doi.org/10.1385/1-59259-750-5:125.Full Text Link to Item
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Keene, Jack D. “Posttranscriptional generation of macromolecular complexes.” Mol Cell 12, no. 6 (December 2003): 1347–49. https://doi.org/10.1016/s1097-2765(03)00496-9.Full Text Link to Item
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Intine, Robert V., Scott A. Tenenbaum, Amy L. Sakulich, Jack D. Keene, and Richard J. Maraia. “Differential phosphorylation and subcellular localization of La RNPs associated with precursor tRNAs and translation-related mRNAs.” Mol Cell 12, no. 5 (November 2003): 1301–7. https://doi.org/10.1016/s1097-2765(03)00429-5.Full Text Link to Item
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Tenenbaum, Scott A., Craig C. Carson, Ulus Atasoy, and Jack D. Keene. “Genome-wide regulatory analysis using en masse nuclear run-ons and ribonomic profiling with autoimmune sera.” Gene 317, no. 1–2 (October 23, 2003): 79–87. https://doi.org/10.1016/s0378-1119(03)00661-9.Full Text Link to Item
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Lerner, Rachel S., Robert M. Seiser, Tianli Zheng, Patrick J. Lager, Mary C. Reedy, Jack D. Keene, and Christopher V. Nicchitta. “Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes.” Rna 9, no. 9 (September 2003): 1123–37. https://doi.org/10.1261/rna.5610403.Full Text Link to Item
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Mazan-Mamczarz, Krystyna, Stefanie Galbán, Isabel López de Silanes, Jennifer L. Martindale, Ulus Atasoy, Jack D. Keene, and Myriam Gorospe. “RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.” Proc Natl Acad Sci U S A 100, no. 14 (July 8, 2003): 8354–59. https://doi.org/10.1073/pnas.1432104100.Full Text Link to Item
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Keene, Jack D. “Organizing mRNA export.” Nat Genet 33, no. 2 (February 2003): 111–12. https://doi.org/10.1038/ng0203-111.Full Text Link to Item
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Keene, Jack D., and Scott A. Tenenbaum. “Eukaryotic mRNPs may represent posttranscriptional operons.” Mol Cell 9, no. 6 (June 2002): 1161–67. https://doi.org/10.1016/s1097-2765(02)00559-2.Full Text Link to Item
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Lipes, Barbara D., and Jack D. Keene. “Autoimmune epitopes in messenger RNA.” Rna 8, no. 6 (June 2002): 762–71. https://doi.org/10.1017/s1355838202021507.Full Text Link to Item
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Eystathioy, Theophany, Edward K. L. Chan, Scott A. Tenenbaum, Jack D. Keene, Kevin Griffith, and Marvin J. Fritzler. “A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles.” Mol Biol Cell 13, no. 4 (April 2002): 1338–51. https://doi.org/10.1091/mbc.01-11-0544.Full Text Link to Item
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Tenenbaum, Scott A., Patrick J. Lager, Craig C. Carson, and Jack D. Keene. “Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays.” Methods 26, no. 2 (February 2002): 191–98. https://doi.org/10.1016/S1046-2023(02)00022-1.Full Text Link to Item
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Brown, V., P. Jin, S. Ceman, J. C. Darnell, W. T. O’Donnell, S. A. Tenenbaum, X. Jin, et al. “Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.” Cell 107, no. 4 (November 16, 2001): 477–87. https://doi.org/10.1016/s0092-8674(01)00568-2.Full Text Link to Item
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Keene, J. D. “Ribonucleoprotein infrastructure regulating the flow of genetic information between the genome and the proteome.” Proc Natl Acad Sci U S A 98, no. 13 (June 19, 2001): 7018–24. https://doi.org/10.1073/pnas.111145598.Full Text Link to Item
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Tenenbaum, S. A., C. C. Carson, P. J. Lager, and J. D. Keene. “Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays.” Proc Natl Acad Sci U S A 97, no. 26 (December 19, 2000): 14085–90. https://doi.org/10.1073/pnas.97.26.14085.Full Text Link to Item
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Ali, N., G. J. Pruijn, D. J. Kenan, J. D. Keene, and A. Siddiqui. “Human La antigen is required for the hepatitis C virus internal ribosome entry site-mediated translation.” J Biol Chem 275, no. 36 (September 8, 2000): 27531–40. https://doi.org/10.1074/jbc.M001487200.Full Text Link to Item
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Nagai, Y., T. Tucker, H. Ren, D. J. Kenan, B. S. Henderson, J. D. Keene, W. J. Strittmatter, and J. R. Burke. “Inhibition of polyglutamine protein aggregation and cell death by novel peptides identified by phage display screening.” J Biol Chem 275, no. 14 (April 7, 2000): 10437–42. https://doi.org/10.1074/jbc.275.14.10437.Full Text Link to Item
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Nagai, Y., T. J. Tucker, D. J. Kenan, B. S. Henderson, J. D. Keene, W. J. Strittmatter, and J. R. Burke. “Identification of QBP1, a synthetic peptide that preferentially binds expanded polyglutamine domain by phage display technique.” American Journal of Human Genetics 65, no. 4 (October 1, 1999): A463–A463.Link to Item
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Antic, D., N. Lu, and J. D. Keene. “ELAV tumor antigen, Hel-N1, increases translation of neurofilament M mRNA and induces formation of neurites in human teratocarcinoma cells.” Genes Dev 13, no. 4 (February 15, 1999): 449–61. https://doi.org/10.1101/gad.13.4.449.Full Text Link to Item
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Ford, L. P., J. Watson, J. D. Keene, and J. Wilusz. “ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.” Genes Dev 13, no. 2 (January 15, 1999): 188–201. https://doi.org/10.1101/gad.13.2.188.Full Text Link to Item
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Keene, J. D. “Why is Hu where? Shuttling of early-response-gene messenger RNA subsets.” Proc Natl Acad Sci U S A 96, no. 1 (January 5, 1999): 5–7. https://doi.org/10.1073/pnas.96.1.5.Full Text Link to Item
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Andrews, L. G., and J. D. Keene. “Identification of specific protein-RNA target sites using libraries of natural sequences.” Methods Mol Biol 118 (1999): 233–44. https://doi.org/10.1385/1-59259-676-2:233.Full Text Link to Item
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Kenan, D. J., and J. D. Keene. “In vitro selection of aptamers from RNA libraries.” Methods Mol Biol 118 (1999): 217–31. https://doi.org/10.1385/1-59259-676-2:217.Full Text Link to Item
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Atasoy, U., J. Watson, D. Patel, and J. D. Keene. “ELAV protein HuA (HuR) can redistribute between nucleus and cytoplasm and is upregulated during serum stimulation and T cell activation.” J Cell Sci 111 ( Pt 21) (November 1998): 3145–56.Link to Item
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Czarnik, A. W., and J. D. Keene. “Combinatorial chemistry.” Curr Biol 8, no. 20 (October 8, 1998): R705–7. https://doi.org/10.1016/s0960-9822(98)70453-1.Full Text Link to Item
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Antic, D., and J. D. Keene. “Messenger ribonucleoprotein complexes containing human ELAV proteins: interactions with cytoskeleton and translational apparatus.” J Cell Sci 111 ( Pt 2) (January 1998): 183–97.Link to Item
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Antic, D., and J. D. Keene. “Embryonic lethal abnormal visual RNA-binding proteins involved in growth, differentiation, and posttranscriptional gene expression.” Am J Hum Genet 61, no. 2 (August 1997): 273–78. https://doi.org/10.1086/514866.Full Text Link to Item
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Jain, R. G., L. G. Andrews, K. M. McGowan, P. H. Pekala, and J. D. Keene. “Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.” Mol Cell Biol 17, no. 2 (February 1997): 954–62. https://doi.org/10.1128/MCB.17.2.954.Full Text Link to Item
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Keene, J. D. “Letters.” Scientist 10, no. 16 (August 19, 1996).
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Keene, J. D. “RNA surfaces as functional mimetics of proteins.” Chem Biol 3, no. 7 (July 1996): 505–13. https://doi.org/10.1016/s1074-5521(96)90139-8.Full Text Link to Item
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Das, S., D. J. Kenan, D. Bocskai, J. D. Keene, and A. Dasgupta. “Sequences within a small yeast RNA required for inhibition of internal initiation of translation: interaction with La and other cellular proteins influences its inhibitory activity.” J Virol 70, no. 3 (March 1996): 1624–32. https://doi.org/10.1128/JVI.70.3.1624-1632.1996.Full Text Link to Item
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Gao, F. B., and J. D. Keene. “Hel-N1/Hel-N2 proteins are bound to poly(A)+ mRNA in granular RNP structures and are implicated in neuronal differentiation.” J Cell Sci 109 ( Pt 3) (March 1996): 579–89.Link to Item
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Keene, J. D. “RNA recognition by autoantigens and autoantibodies.” Mol Biol Rep 23, no. 3–4 (1996): 173–81. https://doi.org/10.1007/BF00351166.Full Text Link to Item
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Keene, J. D. “Randomization and selection of RNA to identify targets for RRM RNA-binding proteins and antibodies.” Methods Enzymol 267 (1996): 367–83. https://doi.org/10.1016/s0076-6879(96)67023-2.Full Text Link to Item
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Methot, N., G. Pickett, J. D. Keene, and N. Sonenberg. “In vitro RNA selection identifies RNA ligands that specifically bind to eukaryotic translation initiation factor 4B: the role of the RNA remotif.” Rna 2, no. 1 (January 1996): 38–50.Link to Item
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Romac, J. M., and J. D. Keene. “Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA.” Genes Dev 9, no. 11 (June 1, 1995): 1400–1410. https://doi.org/10.1101/gad.9.11.1400.Full Text Link to Item
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Chang, Y. N., D. J. Kenan, J. D. Keene, A. Gatignol, and K. T. Jeang. “Direct interactions between autoantigen La and human immunodeficiency virus leader RNA.” J Virol 69, no. 1 (January 1995): 618–19. https://doi.org/10.1128/JVI.69.1.618-619.1995.Full Text Link to Item
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Jain, R. G., L. G. Andrews, K. M. McGowan, F. Gao, J. D. Keene, and P. P. Pekala. “Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR.” Nucleic Acids Symp Ser, no. 33 (1995): 209–11.Link to Item
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Gao, F. B., C. C. Carson, T. Levine, and J. D. Keene. “Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.” Proc Natl Acad Sci U S A 91, no. 23 (November 8, 1994): 11207–11. https://doi.org/10.1073/pnas.91.23.11207.Full Text Link to Item
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Chang, Y. N., D. J. Kenan, J. D. Keene, A. Gatignol, and K. T. Jeang. “Direct interactions between autoantigen La and human immunodeficiency virus leader RNA.” J Virol 68, no. 11 (November 1994): 7008–20. https://doi.org/10.1128/JVI.68.11.7008-7020.1994.Full Text Link to Item
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Romac, J. M., D. H. Graff, and J. D. Keene. “The U1 small nuclear ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain.” Mol Cell Biol 14, no. 7 (July 1994): 4662–70. https://doi.org/10.1128/mcb.14.7.4662-4670.1994.Full Text Link to Item
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Saitta, M. R., F. C. Arnett, and J. D. Keene. “60-kDa Ro protein autoepitopes identified using recombinant polypeptides.” J Immunol 152, no. 8 (April 15, 1994): 4192–4202.Link to Item
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Kelekar, A., M. R. Saitta, and J. D. Keene. “Molecular composition of Ro small ribonucleoprotein complexes in human cells. Intracellular localization of the 60- and 52-kD proteins.” J Clin Invest 93, no. 4 (April 1994): 1637–44. https://doi.org/10.1172/JCI117145.Full Text Link to Item
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King, P. H., T. D. Levine, R. T. Fremeau, and J. D. Keene. “Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor.” J Neurosci 14, no. 4 (April 1994): 1943–52.Link to Item
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Maraia, R. J., D. J. Kenan, and J. D. Keene. “Eukaryotic transcription termination factor La mediates transcript release and facilitates reinitiation by RNA polymerase III.” Mol Cell Biol 14, no. 3 (March 1994): 2147–58. https://doi.org/10.1128/mcb.14.3.2147-2158.1994.Full Text Link to Item
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Kenan, D. J., D. E. Tsai, and J. D. Keene. “Exploring molecular diversity with combinatorial shape libraries.” Trends Biochem Sci 19, no. 2 (February 1994): 57–64. https://doi.org/10.1016/0968-0004(94)90033-7.Full Text Link to Item
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Meerovitch, K., Y. V. Svitkin, H. S. Lee, F. Lejbkowicz, D. J. Kenan, E. K. Chan, V. I. Agol, J. D. Keene, and N. Sonenberg. “La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate.” J Virol 67, no. 7 (July 1993): 3798–3807. https://doi.org/10.1128/JVI.67.7.3798-3807.1993.Full Text Link to Item
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Levine, T. D., F. Gao, P. H. King, L. G. Andrews, and J. D. Keene. “Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.” Mol Cell Biol 13, no. 6 (June 1993): 3494–3504. https://doi.org/10.1128/mcb.13.6.3494-3504.1993.Full Text Link to Item
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Tsai, D. E., and J. D. Keene. “In vitro selection of RNA epitopes using autoimmune patient serum.” J Immunol 150, no. 3 (February 1, 1993): 1137–45.Link to Item
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Tsai, D. E., D. J. Kenan, and J. D. Keene. “In vitro selection of an RNA epitope immunologically cross-reactive with a peptide.” Proc Natl Acad Sci U S A 89, no. 19 (October 1, 1992): 8864–68. https://doi.org/10.1073/pnas.89.19.8864.Full Text Link to Item
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Harper, D. S., L. D. Fresco, and J. D. Keene. “RNA binding specificity of a Drosophila snRNP protein that shares sequence homology with mammalian U1-A and U2-B" proteins.” Nucleic Acids Res 20, no. 14 (July 25, 1992): 3645–50. https://doi.org/10.1093/nar/20.14.3645.Full Text Link to Item
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Saitta, M. R., and J. D. Keene. “Molecular biology of nuclear autoantigens.” Rheum Dis Clin North Am 18, no. 2 (May 1992): 283–310.Link to Item
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Tsai, D. E., D. S. Harper, and J. D. Keene. “U1-snRNP-A protein selects a ten nucleotide consensus sequence from a degenerate RNA pool presented in various structural contexts.” Nucleic Acids Res 19, no. 18 (September 25, 1991): 4931–36. https://doi.org/10.1093/nar/19.18.4931.Full Text Link to Item
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Kenan, D. J., C. C. Query, and J. D. Keene. “RNA recognition: towards identifying determinants of specificity.” Trends Biochem Sci 16, no. 6 (June 1991): 214–20. https://doi.org/10.1016/0968-0004(91)90088-d.Full Text Link to Item
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Bentley, R. C., and J. D. Keene. “Recognition of U1 and U2 small nuclear RNAs can be altered by a 5-amino-acid segment in the U2 small nuclear ribonucleoprotein particle (snRNP) B" protein and through interactions with U2 snRNP-A' protein.” Mol Cell Biol 11, no. 4 (April 1991): 1829–39. https://doi.org/10.1128/mcb.11.4.1829-1839.1991.Full Text Link to Item
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Hoffman, D. W., C. C. Query, B. L. Golden, S. W. White, and J. D. Keene. “RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins.” Proc Natl Acad Sci U S A 88, no. 6 (March 15, 1991): 2495–99. https://doi.org/10.1073/pnas.88.6.2495.Full Text Link to Item
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St Clair, E. W., D. Kenan, J. A. Burch, J. D. Keene, and D. S. Pisetsky. “Anti-La antibody production by MRL-1pr/1pr mice. Analysis of fine specificity.” J Immunol 146, no. 6 (March 15, 1991): 1885–92.Link to Item
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Fresco, L. D., D. S. Harper, and J. D. Keene. “Leucine periodicity of U2 small nuclear ribonucleoprotein particle (snRNP) A' protein is implicated in snRNP assembly via protein-protein interactions.” Mol Cell Biol 11, no. 3 (March 1991): 1578–89. https://doi.org/10.1128/mcb.11.3.1578-1589.1991.Full Text Link to Item
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Tamura, H., C. Kohchi, R. Yamada, T. Ikeda, O. Koiwai, E. Patterson, J. D. Keene, K. Okada, E. Kjeldsen, and K. Nishikawa. “Molecular cloning of a cDNA of a camptothecin-resistant human DNA topoisomerase I and identification of mutation sites.” Nucleic Acids Res 19, no. 1 (January 11, 1991): 69–75. https://doi.org/10.1093/nar/19.1.69.Full Text Link to Item
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Keene, J. D., and C. C. Query. “Nuclear RNA-binding proteins.” Prog Nucleic Acid Res Mol Biol 41 (1991): 179–202. https://doi.org/10.1016/s0079-6603(08)60009-4.Full Text Link to Item
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Sunde, L., E. Kjeldsen, T. Andoh, J. D. Keene, and L. Bolund. “A three allele TaqI polymorphism at TOP1 gene.” Nucleic Acids Res 18, no. 19 (October 11, 1990): 5919. https://doi.org/10.1093/nar/18.19.5919-a.Full Text Link to Item
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Lutz-Freyermuth, C., C. C. Query, and J. D. Keene. “Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA.” Proc Natl Acad Sci U S A 87, no. 16 (August 1990): 6393–97. https://doi.org/10.1073/pnas.87.16.6393.Full Text Link to Item
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St Clair, E. W., D. Kenan, J. A. Burch, J. D. Keene, and D. S. Pisetsky. “The fine specificity of anti-La antibodies induced in mice by immunization with recombinant human La autoantigen.” J Immunol 144, no. 10 (May 15, 1990): 3868–76.Link to Item
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St Clair, E. W., C. C. Query, R. Bentley, J. D. Keene, R. P. Polisson, N. B. Allen, D. S. Caldwell, J. R. Rice, C. Cox, and D. S. Pisetsky. “Expression of autoantibodies to recombinant (U1) RNP-associated 70K antigen in systemic lupus erythematosus.” Clin Immunol Immunopathol 54, no. 2 (February 1990): 266–80. https://doi.org/10.1016/0090-1229(90)90088-8.Full Text Link to Item
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St Clair, E. W., J. A. Burch, M. M. Ward, J. D. Keene, and D. S. Pisetsky. “Temporal correlation of antibody responses to different epitopes of the human La autoantigen.” J Clin Invest 85, no. 2 (February 1990): 515–21. https://doi.org/10.1172/JCI114467.Full Text Link to Item
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Harper, D. S., L. D. Fresco, and J. D. Keene. “Characterization of a Drosophila U snRNP RNA-binding protein.” Mol Biol Rep 14, no. 2–3 (1990): 163. https://doi.org/10.1007/BF00360458.Full Text Link to Item
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James, J. A., W. D. Dickey, A. Fujisaku, C. A. O’Brien, S. L. Deutscher, J. D. Keene, and J. B. Harley. “Antigenicity of a recombinant Ro (SS-A) fusion protein.” Arthritis Rheum 33, no. 1 (January 1990): 102–6. https://doi.org/10.1002/art.1780330114.Full Text Link to Item
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Kelekar, A., and J. D. Keene. “Downregulation of RNA polymerase III transcription of the hY3 gene in vitro.” Mol Biol Rep 14, no. 2–3 (1990): 173–74. https://doi.org/10.1007/BF00360463.Full Text Link to Item
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Query, C. C., R. C. Bentley, and J. D. Keene. “A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component.” Mol Cell Biol 9, no. 11 (November 1989): 4872–81. https://doi.org/10.1128/mcb.9.11.4872-4881.1989.Full Text Link to Item
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Crone, D. E., and J. D. Keene. “Viral transcription is necessary and sufficient for vesicular stomatitis virus to inhibit maturation of small nuclear ribonucleoproteins.” J Virol 63, no. 10 (October 1989): 4172–80. https://doi.org/10.1128/JVI.63.10.4172-4180.1989.Full Text Link to Item
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Frielle, D. W., P. B. Kim, and J. D. Keene. “Inhibitory effects of vesicular stomatitis virus on cellular and influenza viral RNA metabolism and protein synthesis.” Virology 172, no. 1 (September 1989): 274–84. https://doi.org/10.1016/0042-6822(89)90129-3.Full Text Link to Item
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Keene, J. D. “Molecular structure of the La and Ro autoantigens and their use in autoimmune diagnostics.” J Autoimmun 2, no. 4 (August 1989): 329–34. https://doi.org/10.1016/0896-8411(89)90160-1.Full Text Link to Item
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St Clair, E. W., N. Talal, H. M. Moutsopoulos, A. Ballester, L. Zerva, J. D. Keene, and D. S. Pisetsky. “Epitope specificity of anti-La antibodies from patients with Sjögren's syndrome.” J Autoimmun 2, no. 4 (August 1989): 335–44. https://doi.org/10.1016/0896-8411(89)90161-3.Full Text Link to Item
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Lutz-Freyermuth, C., and J. D. Keene. “The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.” Mol Cell Biol 9, no. 7 (July 1989): 2975–82. https://doi.org/10.1128/mcb.9.7.2975-2982.1989.Full Text Link to Item
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Query, C. C., R. C. Bentley, and J. D. Keene. “A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.” Cell 57, no. 1 (April 7, 1989): 89–101. https://doi.org/10.1016/0092-8674(89)90175-x.Full Text Link to Item
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St Clair, E. W., D. S. Pisetsky, C. F. Reich, and J. D. Keene. “Analysis of autoantibody binding to different regions of the human La antigen expressed in recombinant fusion proteins.” J Immunol 141, no. 12 (December 15, 1988): 4173–80.Link to Item
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Chambers, J. C., D. Kenan, B. J. Martin, and J. D. Keene. “Genomic structure and amino acid sequence domains of the human La autoantigen.” J Biol Chem 263, no. 34 (December 5, 1988): 18043–51.Link to Item
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Deutscher, S. L., J. B. Harley, and J. D. Keene. “Molecular analysis of the 60-kDa human Ro ribonucleoprotein.” Proc Natl Acad Sci U S A 85, no. 24 (December 1988): 9479–83. https://doi.org/10.1073/pnas.85.24.9479.Full Text Link to Item
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Deutscher, S. L., and J. D. Keene. “A sequence-specific conformational epitope on U1 RNA is recognized by a unique autoantibody.” Proc Natl Acad Sci U S A 85, no. 10 (May 1988): 3299–3303. https://doi.org/10.1073/pnas.85.10.3299.Full Text Link to Item
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St Clair, E. W., D. S. Pisetsky, C. F. Reich, J. C. Chambers, and J. D. Keene. “Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen.” Arthritis Rheum 31, no. 4 (April 1988): 506–14. https://doi.org/10.1002/art.1780310407.Full Text Link to Item
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Query, C. C., and J. D. Keene. “A human autoimmune protein associated with U1 RNA contains a region of homology that is cross-reactive with retroviral p30gag antigen.” Cell 51, no. 2 (October 23, 1987): 211–20. https://doi.org/10.1016/0092-8674(87)90148-6.Full Text Link to Item
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Chambers, J. C., and J. D. Keene. “Association between autoimmune epitopes and RNA binding domains of the human Lupus La protein.” Molecular Biology Reports 12, no. 3 (September 1, 1987): 241. https://doi.org/10.1007/BF00356919.Full Text
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Deutscher, S. L., J. B. Harley, and J. D. Keene. “Molecular characterization of the Ro small cytoplasmic RNPS.” Molecular Biology Reports 12, no. 3 (September 1, 1987): 242. https://doi.org/10.1007/BF00356920.Full Text
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Fresco, L. D., and J. D. Keene. “Molecular analysis of the U2 snRNP unique protein A′.” Molecular Biology Reports 12, no. 3 (September 1, 1987): 155. https://doi.org/10.1007/BF00356873.Full Text
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Keene, J. D., S. L. Deutscher, D. Kenan, and A. Kelekar. “Nature of the la and ro RNPs.” Molecular Biology Reports 12, no. 3 (September 1, 1987): 235–38. https://doi.org/10.1007/BF00356917.Full Text
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Query, C. C., and J. D. Keene. “The U1 RNA-specific 70K protein: RNA-binding studies and autoimmune cross-reactivity with type-C retroviruses.” Molecular Biology Reports 12, no. 3 (September 1, 1987): 158. https://doi.org/10.1007/BF00356875.Full Text
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Fresco, L. D., M. G. Kurilla, and J. D. Keene. “Rapid inhibition of processing and assembly of small nuclear ribonucleoproteins after infection with vesicular stomatitis virus.” Mol Cell Biol 7, no. 3 (March 1987): 1148–55. https://doi.org/10.1128/mcb.7.3.1148-1155.1987.Full Text Link to Item
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Wilusz, J., and J. D. Keene. “Autoantibodies specific for U1 RNA and initiator methionine tRNA.” J Biol Chem 261, no. 12 (April 25, 1986): 5467–72.Link to Item
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Kiley, M. P., J. Wilusz, J. B. McCormick, and J. D. Keene. “Conservation of the 3' terminal nucleotide sequences of Ebola and Marburg virus.” Virology 149, no. 2 (March 1986): 251–54. https://doi.org/10.1016/0042-6822(86)90127-3.Full Text Link to Item
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Kole, R., L. D. Fresco, J. D. Keene, P. L. Cohen, R. A. Eisenberg, and P. G. Andrews. “Alu RNA-protein complexes formed in vitro react with a novel lupus autoantibody.” Journal of Biological Chemistry 260, no. 21 (December 1, 1985): 11781–86.
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Pisetsky, D. S., S. O. Hoch, C. L. Klatt, M. A. O’Donnell, and J. D. Keene. “Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity.” J Immunol 135, no. 6 (December 1985): 4080–85.Link to Item
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Piwnica-Worms, H., and J. D. Keene. “Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells.” Journal of Biological Chemistry 260, no. 19 (December 1, 1985): 10503–11.
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Kole, R., L. D. Fresco, J. D. Keene, P. L. Cohen, R. A. Eisenberg, and P. G. Andrews. “Alu RNA-protein complexes formed in vitro react with a novel lupus autoantibody.” J Biol Chem 260, no. 21 (September 25, 1985): 11781–86.Link to Item
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Piwnica-Worms, H., and J. D. Keene. “Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells.” J Biol Chem 260, no. 19 (September 5, 1985): 10503–11.Link to Item
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Kurilla, M. G., H. O. Stone, and J. D. Keene. “RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome.” Virology 145, no. 2 (September 1985): 203–12. https://doi.org/10.1016/0042-6822(85)90154-0.Full Text Link to Item
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Keene, J. D. “Interactions between small viral RNAs of vesicular stomatitis virus and components of cellular gene expression.” Microbiol Sci 2, no. 5 (May 1985): 152–56.Link to Item
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Chambers, J. C., and J. D. Keene. “Isolation and analysis of cDNA clones expressing human lupus La antigen.” Proc Natl Acad Sci U S A 82, no. 7 (April 1985): 2115–19. https://doi.org/10.1073/pnas.82.7.2115.Full Text Link to Item
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Wilusz, J., J. S. Youngner, and J. D. Keene. “Base mutations in the terminal noncoding regions of the genome of vesicular stomatitis virus isolated from persistent infections of L cells.” Virology 140, no. 2 (January 30, 1985): 249–56. https://doi.org/10.1016/0042-6822(85)90363-0.Full Text Link to Item
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Meier, E., G. G. Harmison, J. D. Keene, and M. Schubert. “Sites of copy choice replication involved in generation of vesicular stomatitis virus defective-interfering particle RNAs.” J Virol 51, no. 2 (August 1984): 515–21. https://doi.org/10.1128/JVI.51.2.515-521.1984.Full Text Link to Item
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Kurilla, M. G., C. D. Cabradilla, B. P. Holloway, and J. D. Keene. “Nucleotide sequence and host La protein interactions of rabies virus leader RNA.” J Virol 50, no. 3 (June 1984): 773–78. https://doi.org/10.1128/JVI.50.3.773-778.1984.Full Text Link to Item
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Wilusz, J., and J. D. Keene. “Interactions of plus and minus strand leader RNAs of the New Jersey serotype of vesicular stomatitis virus with the cellular La protein.” Virology 135, no. 1 (May 1984): 65–73. https://doi.org/10.1016/0042-6822(84)90117-x.Full Text Link to Item
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Chambers, J. C., M. G. Kurilla, and J. D. Keene. “Association between the 7 S RNA and the lupus La protein varies among cell types.” J Biol Chem 258, no. 19 (October 10, 1983): 11438–41.Link to Item
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Kurilla, M. G., and J. D. Keene. “The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.” Cell 34, no. 3 (October 1983): 837–45. https://doi.org/10.1016/0092-8674(83)90541-x.Full Text Link to Item
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Wilusz, J., M. G. Kurilla, and J. D. Keene. “A host protein (La) binds to a unique species of minus-sense leader RNA during replication of vesicular stomatitis virus.” Proc Natl Acad Sci U S A 80, no. 19 (October 1983): 5827–31. https://doi.org/10.1073/pnas.80.19.5827.Full Text Link to Item
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Piwnica-Worms, H., and J. D. Keene. “Sequential synthesis of small capped RNA transcripts in vitro by vesicular stomatitis virus.” Virology 125, no. 1 (February 1983): 206–18. https://doi.org/10.1016/0042-6822(83)90074-0.Full Text Link to Item
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Chambers, J. C., M. G. Kurilla, and J. D. Keene. “Association between the 7 S RNA and the lupus La protein varies among cell types.” Journal of Biological Chemistry 258, no. 19 (January 1, 1983): 11438–41.
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Gaillard, R. K., J. K. Li, J. D. Keene, and W. K. Joklik. “The sequence at the termini of four genes of the three reovirus serotypes.” Virology 121, no. 2 (September 1982): 320–26. https://doi.org/10.1016/0042-6822(82)90171-4.Full Text Link to Item
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Kurilla, M. G., H. Piwnica-Worms, and J. D. Keene. “Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells.” Proc Natl Acad Sci U S A 79, no. 17 (September 1982): 5240–44. https://doi.org/10.1073/pnas.79.17.5240.Full Text Link to Item
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Isaac, C. L., and J. D. Keene. “RNA polymerase-associated interactions near template promoter sequences of defective interfering particles of vesicular stomatitis virus.” J Virol 43, no. 1 (July 1982): 241–49. https://doi.org/10.1128/JVI.43.1.241-249.1982.Full Text Link to Item
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Lazzarini, R. A., I. Chien, F. Yang, and J. D. Keene. “The metabolic fate of independently initiated VSV mRNA transcripts.” J Gen Virol 58, no. Pt 2 (February 1982): 429–41. https://doi.org/10.1099/0022-1317-58-2-429.Full Text Link to Item
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Schmidt, E. V., J. D. Keene, M. Linial, and R. E. Smith. “Association of 3' terminal RNA sequences with avian leukosis viruses causing a high incidence of osteopetrosis.” Virology 116, no. 1 (January 15, 1982): 163–80. https://doi.org/10.1016/0042-6822(82)90411-1.Full Text Link to Item
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Keene, J. D., B. J. Thornton, and S. U. Emerson. “Sequence-specific contacts between the RNA polymerase of vesicular stomatitis virus and the leader RNA gene.” Proc Natl Acad Sci U S A 78, no. 10 (October 1981): 6191–95. https://doi.org/10.1073/pnas.78.10.6191.Full Text Link to Item
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Lazzarini, R. A., J. D. Keene, and M. Schubert. “The origins of defective interfering particles of the negative-strand RNA viruses.” Cell 26, no. 2 Pt 2 (October 1981): 145–54. https://doi.org/10.1016/0092-8674(81)90298-1.Full Text Link to Item
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Isaac, C. L., and J. D. Keene. “Transfer RNAs associated with vesicular stomatitis virus.” J Gen Virol 56, no. Pt 1 (September 1981): 141–51. https://doi.org/10.1099/0022-1317-56-1-141.Full Text Link to Item
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Keene, J. D., I. M. Chien, and R. A. Lazzarini. “Vesicular stomatitis virus defective interfering particle containing a muted internal leader RNA gene.” Proc Natl Acad Sci U S A 78, no. 4 (April 1981): 2090–94. https://doi.org/10.1073/pnas.78.4.2090.Full Text Link to Item
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Herman, R. C., M. Schubert, J. D. Keene, and R. A. Lazzarini. “Polycistronic vesicular stomatitis virus RNA transcripts.” Proc Natl Acad Sci U S A 77, no. 8 (August 1980): 4662–65. https://doi.org/10.1073/pnas.77.8.4662.Full Text Link to Item
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Li, J. K., J. D. Keene, P. P. Scheible, and W. K. Joklik. “Nature of the 3'-terminal sequences of the plus and minus strands of the S1 gene of reovirus serotypes 1, 2 and 3.” Virology 105, no. 1 (August 1980): 41–51. https://doi.org/10.1016/0042-6822(80)90154-3.Full Text Link to Item
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Li, J. K., P. P. Scheible, J. D. Keene, and W. K. Joklik. “The plus strand of reovirus gene S2 is identical with its in vitro transcript.” Virology 105, no. 1 (August 1980): 282–86. https://doi.org/10.1016/0042-6822(80)90181-6.Full Text Link to Item
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Schubert, M., J. D. Keene, R. C. Herman, and R. A. Lazzarini. “Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA.” J Virol 34, no. 2 (May 1980): 550–59. https://doi.org/10.1128/JVI.34.2.550-559.1980.Full Text Link to Item
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Keene, J. D., M. Schubert, and R. A. Lazzarini. “Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA.” J Virol 33, no. 2 (February 1980): 789–94. https://doi.org/10.1128/JVI.33.2.789-794.1980.Full Text Link to Item
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Schubert, M., J. D. Keene, and R. A. Lazzarini. “A specific internal RNA polymerase recognition site of VSV RNA is involved in the generation of DI particles.” Cell 18, no. 3 (November 1979): 749–57. https://doi.org/10.1016/0092-8674(79)90128-4.Full Text Link to Item
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Keene, J. D., M. Schubert, and R. A. Lazzarini. “Terminal sequences of vesicular stomatitis virus RNA are both complementary and conserved.” J Virol 32, no. 1 (October 1979): 167–74. https://doi.org/10.1128/JVI.32.1.167-174.1979.Full Text Link to Item
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Schubert, M., J. D. Keene, R. A. Lazzarini, and S. U. Emerson. “The complete sequence of a unique RNA species synthesized by a DI particle of VSV.” Cell 15, no. 1 (September 1978): 103–12. https://doi.org/10.1016/0092-8674(78)90086-7.Full Text Link to Item
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Keene, J. D., M. Schubert, R. A. Lazzarini, and M. Rosenberg. “Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles.” Proc Natl Acad Sci U S A 75, no. 7 (July 1978): 3225–29. https://doi.org/10.1073/pnas.75.7.3225.Full Text Link to Item
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Colonno, R. J., R. A. Lazzarini, J. D. Keene, and A. K. Banerjee. “In vitro synthesis of messenger RNA by a defective interfering particle of vesicular stomatitis virus.” Proc Natl Acad Sci U S A 74, no. 5 (May 1977): 1884–88. https://doi.org/10.1073/pnas.74.5.1884.Full Text Link to Item
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Keene, J. D., M. Rosenberg, and R. A. Lazzarini. “Characterization of the 3' terminus of RNA isolated from vesicular stomatitis virus and from its defective interfering particles.” Proc Natl Acad Sci U S A 74, no. 4 (April 1977): 1353–57. https://doi.org/10.1073/pnas.74.4.1353.Full Text Link to Item
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Keene, J. D., and R. A. Lazzarini. “A comparison of the extents of methylation of vesicular stomatitis virus messenger RNA.” Virology 69, no. 1 (January 1976): 364–67. https://doi.org/10.1016/0042-6822(76)90229-4.Full Text Link to Item
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Book Sections
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Keene, J. D. “Preface,” Vol. 768, 2013. https://doi.org/10.1007/978-1-4614-5107_5.Full Text
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Keene, Jack D. “Ten Years of Progress in GW/P Body Research Preface.” In TEN YEARS OF PROGRESS IN GW/P BODY RESEARCH, edited by E. K. L. Chan and M. J. Fritzler, 768:V–VI. SPRINGER, 2013.Link to Item
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Conference Papers
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Dutta, Rajesh Kumar, Matthew B. Friedersdorf, Jack D. Keene, Perry J. Blackshear, and Anna Mae Diehl. “THE RNA BINDING PROTEIN ZFP36L1 MAINTAINS HEPATOCYTE MATURITY IN HEALTHY ADULT LIVER.” In Hepatology, 72:246A-246A, 2020.Link to Item
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Simone Bisogno, Laura, and Jack D. Keene. “Abstract P6-07-06: Post-transcriptional coordination of gene expression during breast cancer tumorigenesis.” In Poster Session Abstracts. American Association for Cancer Research, 2015. https://doi.org/10.1158/1538-7445.sabcs14-p6-07-06.Full Text
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Keene, J. D., and Cold Spring Harbor Lab Press. “Biological Clocks and the Coordination Theory of RNA Operons and Regulons.” In Clocks and Rhythms, 72:157–65, 2007.Link to Item
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MESSINA, J. P., D. J. KENAN, J. D. KEENE, and D. S. PISETSKY. “INVITRO SELECTION OF DOUBLE-STRANDED DNA-MOLECULES THAT BIND AN ANTI-DNA ANTIBODY.” In Arthritis and Rheumatism, 35:S72–S72. LIPPINCOTT-RAVEN PUBL, 1992.Link to Item
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STCLAIR, E. W., D. KENAN, J. A. BURCH, J. D. KEENE, and D. S. PISETSKY. “SPONTANEOUS ANTI-LA ANTIBODY-PRODUCTION BY MRL-LPR/LPR MICE.” In Clinical Research, 38:A459–A459. SLACK INC, 1990.Link to Item
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STCLAIR, E. W., D. KENAN, J. A. BURCH, J. D. KEENE, and D. S. PISETSKY. “THE FINE SPECIFICITY OF ANTI-LA ANTIBODIES INDUCED IN MICE BY IMMUNIZATION WITH RECOMBINANT HUMAN LA AUTOANTIGEN.” In Clinical Research, 38:A365–A365. SLACK INC, 1990.Link to Item
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DICKEY, W. D., K. W. JACKSON, S. L. DEUTSCHER, J. D. KEENE, and J. B. HARLEY. “A CARBOXY TERMINAL AUTOEPITOPE OF RO SSA.” In Clinical Research, 37:A488–A488. SLACK INC, 1989.Link to Item
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STCLAIR, E. W., D. S. PISETSKY, J. A. BURCH, and J. D. KEENE. “VARIATIONS IN SERUM ANTIBODY-LEVELS TO LA EPITOPES ON RECOMBINANT FUSION PROTEINS.” In Clinical Research, 37:A588–A588. SLACK INC, 1989.Link to Item
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STCLAIR, E. W., C. C. QUERY, D. S. PISETSKY, and J. D. KEENE. “LUPUS SERA FREQUENTLY RECOGNIZE CLONED U1 RNP-ASSOCIATED 70 K FUSION PROTEIN.” In Clinical Research, 36:A600–A600. SLACK INC, 1988.Link to Item
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STCLAIR, E. W., D. S. PISETSKY, J. C. CHAMBERS, C. F. REICH, and J. D. KEENE. “HUMAN AUTOANTIBODIES RECOGNIZE MULTIPLE EPITOPES ON RECOMBINANT LA FUSION PROTEINS.” In Clinical Research, 36:A537–A537. SLACK INC, 1988.Link to Item
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- Teaching & Mentoring
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Recent Courses
- MGM 293: Research Independent Study I 2021
- MGM 293: Research Independent Study I 2020
- BIOLOGY 728C: University Program in Genetics and Genomics Biological Solutions Module Ill 2019
- CMB 778C: University Program in Genetics and Genomics Biological Solutions Module Ill 2019
- MGM 293: Research Independent Study I 2019
- MGM 593: Research Independent Study 2019
- MGM 778C: University Program in Genetics and Genomics Biological Solutions Module Ill 2019
- UPGEN 778C: University Program in Genetics and Genomics Biological Solutions Module Ill 2019
- Scholarly, Clinical, & Service Activities
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Service to the Profession
- Board Member. Transdisciplinary Centers Advisory Committee. March of Dimes. 2013 - 2018 2013 - 2018
- Organizer. RNA Regulatory Mechanisms Implicated in Human Diseases, both Rare and Common. RNA Stability Group. 2010 2010
- Chair, Board of Scientific Counselors. National Institutes of Health. National Institute of Environmental Health Sciences. 2009 - 2013 2009 - 2013
- Member. Forum on Drug Discovery, Development and Translation. Institute of Medicine, National Academy of Medicine. 2009 - 2015 2009 - 2015
- Reviewer. Systems Biology of Complex Diseases. Helmholtz Association. 2006 2006
- Co-organizer. Systems Biology: Global Regulation of Gene Expression. Cold Spring Harbor Meetings. 2005 - 2006 2005 - 2006
- Member. National Selection and Advisory Board. PEW Scholars in the Biomedical Sciences. 1991 - 1996 1991 - 1996
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Service to Duke
Some information on this profile has been compiled automatically from Duke databases and external sources. (Our About page explains how this works.) If you see a problem with the information, please write to Scholars@Duke and let us know. We will reply promptly.
