Successful immunization with a single injection of non-integrating lentiviral vector.

Journal Article

We evaluated the ability of an integrase (IN)-defective self-inactivating lentiviral vector (sinLV) for the delivery of human immunodeficiency virus-1 (HIV-1) envelope sequences in mice to elicit specific immune responses. BALB/c mice were immunized with a single intramuscular injection of the IN-defective sinLV expressing the codon optimized HIV-1(JR-FL) gp120 sequence, and results were compared with those for the IN-competent counterpart. The IN-defective sinLV elicited specific and long-lasting immune responses, as evaluated up to 90 days from the immunization by enzyme-linked immunosorbent spot (ELISPOT) and intracellular staining (ICS) for interferon-gamma (IFN-gamma) assays in both splenocytes and bone marrow (BM) cells, chromium release assay in splenocytes, and antibody detection in sera, without integration of the vector into the host genome. These data provide evidence that a single administration of an IN-defective sinLV elicits a significant immune response in the absence of vector integration and may be a safe and useful strategy for vaccine development.

Full Text

Duke Authors

Cited Authors

  • Negri, DRM; Michelini, Z; Baroncelli, S; Spada, M; Vendetti, S; Buffa, V; Bona, R; Leone, P; Klotman, ME; Cara, A

Published Date

  • September 2007

Published In

Volume / Issue

  • 15 / 9

Start / End Page

  • 1716 - 1723

PubMed ID

  • 17593926

International Standard Serial Number (ISSN)

  • 1525-0016

Digital Object Identifier (DOI)

  • 10.1038/sj.mt.6300241

Language

  • eng

Conference Location

  • United States