Retroviral E-DNA: persistence and gene expression in nondividing immune cells.

Journal Article (Journal Article;Review)

Following retroviral infection of cells, not only is the proviral DNA integrated into the host genome, but there is also an accumulation of unintegrated extrachromosomal DNA (E-DNA), both linear and circular. Although the integrated DNA is responsible for the production of viral proteins and new viral progeny, the role of E-DNA has remained uncertain. Several reports have shown that E-DNA is transcriptionally active producing both RNA, as well as viral proteins and that circular E-DNA can persist in nondividing cells, raising questions regarding the potential consequences of this reservoir. Furthermore, integrase inhibitors, presently in clinical trials, shifts the balance of proviral DNA to the E-DNA form. This review is focused on recent work in this field with an emphasis on exploring the potential role of E-DNA in both pathogenesis of retroviral infections, especially HIV-1, and as a tool to deliver and express genes.

Full Text

Duke Authors

Cited Authors

  • Cara, A; Klotman, ME

Published Date

  • November 2006

Published In

Volume / Issue

  • 80 / 5

Start / End Page

  • 1013 - 1017

PubMed ID

  • 16923918

International Standard Serial Number (ISSN)

  • 0741-5400

Digital Object Identifier (DOI)

  • 10.1189/jlb.0306151

Language

  • eng

Conference Location

  • United States