Obesity, insulin resistance, and skeletal muscle nitric oxide synthase.

Journal Article (Journal Article)

The molecular mechanisms responsible for impaired insulin action have yet to be fully identified. Rodent models demonstrate a strong relationship between insulin resistance and an elevation in skeletal muscle inducible nitric oxide synthase (iNOS) expression; the purpose of this investigation was to explore this potential relationship in humans. Sedentary men and women were recruited to participate (means ± SE: nonobese, body mass index = 25.5 ± 0.3 kg/m(2), n = 13; obese, body mass index = 36.6 ± 0.4 kg/m(2), n = 14). Insulin sensitivity was measured using an intravenous glucose tolerance test with the subsequent modeling of an insulin sensitivity index (S(I)). Skeletal muscle was obtained from the vastus lateralis, and iNOS, endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) content were determined by Western blot. S(I) was significantly lower in the obese compared with the nonobese group (~43%; P < 0.05), yet skeletal muscle iNOS protein expression was not different between nonobese and obese groups. Skeletal muscle eNOS protein was significantly higher in the nonobese than the obese group, and skeletal muscle nNOS protein tended to be higher (P = 0.054) in the obese compared with the nonobese group. Alternative analysis based on S(I) (high and low tertile) indicated that the most insulin-resistant group did not have significantly more skeletal muscle iNOS protein than the most insulin-sensitive group. In conclusion, human insulin resistance does not appear to be associated with an elevation in skeletal muscle iNOS protein in middle-aged individuals under fasting conditions.

Full Text

Duke Authors

Cited Authors

  • Kraus, RM; Houmard, JA; Kraus, WE; Tanner, CJ; Pierce, JR; Choi, MD; Hickner, RC

Published Date

  • September 1, 2012

Published In

Volume / Issue

  • 113 / 5

Start / End Page

  • 758 - 765

PubMed ID

  • 22797309

Pubmed Central ID

  • PMC3472472

Electronic International Standard Serial Number (EISSN)

  • 1522-1601

Digital Object Identifier (DOI)

  • 10.1152/japplphysiol.01018.2011


  • eng

Conference Location

  • United States