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Obesity, insulin resistance, and skeletal muscle nitric oxide synthase.

Publication ,  Journal Article
Kraus, RM; Houmard, JA; Kraus, WE; Tanner, CJ; Pierce, JR; Choi, MD; Hickner, RC
Published in: J Appl Physiol (1985)
September 1, 2012

The molecular mechanisms responsible for impaired insulin action have yet to be fully identified. Rodent models demonstrate a strong relationship between insulin resistance and an elevation in skeletal muscle inducible nitric oxide synthase (iNOS) expression; the purpose of this investigation was to explore this potential relationship in humans. Sedentary men and women were recruited to participate (means ± SE: nonobese, body mass index = 25.5 ± 0.3 kg/m(2), n = 13; obese, body mass index = 36.6 ± 0.4 kg/m(2), n = 14). Insulin sensitivity was measured using an intravenous glucose tolerance test with the subsequent modeling of an insulin sensitivity index (S(I)). Skeletal muscle was obtained from the vastus lateralis, and iNOS, endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) content were determined by Western blot. S(I) was significantly lower in the obese compared with the nonobese group (~43%; P < 0.05), yet skeletal muscle iNOS protein expression was not different between nonobese and obese groups. Skeletal muscle eNOS protein was significantly higher in the nonobese than the obese group, and skeletal muscle nNOS protein tended to be higher (P = 0.054) in the obese compared with the nonobese group. Alternative analysis based on S(I) (high and low tertile) indicated that the most insulin-resistant group did not have significantly more skeletal muscle iNOS protein than the most insulin-sensitive group. In conclusion, human insulin resistance does not appear to be associated with an elevation in skeletal muscle iNOS protein in middle-aged individuals under fasting conditions.

Duke Scholars

Published In

J Appl Physiol (1985)

DOI

EISSN

1522-1601

Publication Date

September 1, 2012

Volume

113

Issue

5

Start / End Page

758 / 765

Location

United States

Related Subject Headings

  • Physiology
  • Obesity
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase Type I
  • Nitric Oxide Synthase
  • Muscle, Skeletal
  • Middle Aged
  • Male
  • Insulin Resistance
 

Citation

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Kraus, R. M., Houmard, J. A., Kraus, W. E., Tanner, C. J., Pierce, J. R., Choi, M. D., & Hickner, R. C. (2012). Obesity, insulin resistance, and skeletal muscle nitric oxide synthase. J Appl Physiol (1985), 113(5), 758–765. https://doi.org/10.1152/japplphysiol.01018.2011
Kraus, Raymond M., Joseph A. Houmard, William E. Kraus, Charles J. Tanner, Joseph R. Pierce, Myung Dong Choi, and Robert C. Hickner. “Obesity, insulin resistance, and skeletal muscle nitric oxide synthase.J Appl Physiol (1985) 113, no. 5 (September 1, 2012): 758–65. https://doi.org/10.1152/japplphysiol.01018.2011.
Kraus RM, Houmard JA, Kraus WE, Tanner CJ, Pierce JR, Choi MD, et al. Obesity, insulin resistance, and skeletal muscle nitric oxide synthase. J Appl Physiol (1985). 2012 Sep 1;113(5):758–65.
Kraus, Raymond M., et al. “Obesity, insulin resistance, and skeletal muscle nitric oxide synthase.J Appl Physiol (1985), vol. 113, no. 5, Sept. 2012, pp. 758–65. Pubmed, doi:10.1152/japplphysiol.01018.2011.
Kraus RM, Houmard JA, Kraus WE, Tanner CJ, Pierce JR, Choi MD, Hickner RC. Obesity, insulin resistance, and skeletal muscle nitric oxide synthase. J Appl Physiol (1985). 2012 Sep 1;113(5):758–765.

Published In

J Appl Physiol (1985)

DOI

EISSN

1522-1601

Publication Date

September 1, 2012

Volume

113

Issue

5

Start / End Page

758 / 765

Location

United States

Related Subject Headings

  • Physiology
  • Obesity
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase Type I
  • Nitric Oxide Synthase
  • Muscle, Skeletal
  • Middle Aged
  • Male
  • Insulin Resistance