A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.

Journal Article (Journal Article)

The ultrastructural localization of B-1,3-glucanase in three species of sea urchin eggs was determined using a monospecific antibody in an electronmicroscopic immunogold procedure. In all three species, Lytechinus variegatus, Strongylocentrotus purpuratus, and Arbacia punctulata, B-1,3-glucanase was localized specifically to the cortical granules. No other organelle within the egg contained significant label. During the fertilization reaction, B-1,3-glucanase was released from cortical granules into the perivitelline space and became associated with the hyaline layer. No significant label was found in association with the fertilization envelope.

Full Text

Duke Authors

Cited Authors

  • Wessel, GM; Truschel, MR; Chambers, SA; McClay, DR

Published Date

  • December 1987

Published In

Volume / Issue

  • 18 / 4

Start / End Page

  • 339 - 348

PubMed ID

  • 3148543

International Standard Serial Number (ISSN)

  • 0148-7280

Digital Object Identifier (DOI)

  • 10.1002/mrd.1120180408


  • eng