A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.
Publication
, Journal Article
Wessel, GM; Truschel, MR; Chambers, SA; McClay, DR
Published in: Gamete research
December 1987
The ultrastructural localization of B-1,3-glucanase in three species of sea urchin eggs was determined using a monospecific antibody in an electronmicroscopic immunogold procedure. In all three species, Lytechinus variegatus, Strongylocentrotus purpuratus, and Arbacia punctulata, B-1,3-glucanase was localized specifically to the cortical granules. No other organelle within the egg contained significant label. During the fertilization reaction, B-1,3-glucanase was released from cortical granules into the perivitelline space and became associated with the hyaline layer. No significant label was found in association with the fertilization envelope.
Duke Scholars
Published In
Gamete research
DOI
ISSN
0148-7280
Publication Date
December 1987
Volume
18
Issue
4
Start / End Page
339 / 348
Related Subject Headings
- beta-Glucosidase
- Species Specificity
- Sea Urchins
- Ovum
- Microscopy, Electron
- Immunohistochemistry
- Glucosidases
- Glucan 1,3-beta-Glucosidase
- Female
- Cytoplasmic Granules
Citation
APA
Chicago
ICMJE
MLA
NLM
Wessel, G. M., Truschel, M. R., Chambers, S. A., & McClay, D. R. (1987). A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs. Gamete Research, 18(4), 339–348. https://doi.org/10.1002/mrd.1120180408
Wessel, G. M., M. R. Truschel, S. A. Chambers, and D. R. McClay. “A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.” Gamete Research 18, no. 4 (December 1987): 339–48. https://doi.org/10.1002/mrd.1120180408.
Wessel GM, Truschel MR, Chambers SA, McClay DR. A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs. Gamete research. 1987 Dec;18(4):339–48.
Wessel, G. M., et al. “A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.” Gamete Research, vol. 18, no. 4, Dec. 1987, pp. 339–48. Epmc, doi:10.1002/mrd.1120180408.
Wessel GM, Truschel MR, Chambers SA, McClay DR. A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs. Gamete research. 1987 Dec;18(4):339–348.
Published In
Gamete research
DOI
ISSN
0148-7280
Publication Date
December 1987
Volume
18
Issue
4
Start / End Page
339 / 348
Related Subject Headings
- beta-Glucosidase
- Species Specificity
- Sea Urchins
- Ovum
- Microscopy, Electron
- Immunohistochemistry
- Glucosidases
- Glucan 1,3-beta-Glucosidase
- Female
- Cytoplasmic Granules