A multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in Saccharomyces cerevisiae.

Published

Journal Article

A multispecies-based taxonomic microarray targeting coding sequences of diverged orthologous genes in Saccharomyces cerevisiae, Saccharomyces paradoxus, Saccharomyces mikatae, Saccharomyces bayanus, Saccharomyces kudriavzevii, Naumovia castellii, Lachancea kluyveri and Candida glabrata was designed to allow identification of isolates of these species and their interspecies hybrids. Analysis of isolates of several Saccharomyces species and interspecies hybrids demonstrated the ability of the microarray to differentiate these yeasts on the basis of their specific hybridization patterns. Subsequent analysis of 183 supposed S. cerevisiae isolates of various ecological and geographical backgrounds revealed one misclassified S. bayanus or Saccharomyces uvarum isolate and four aneuploid interspecies hybrids, one between S. cerevisiae and S. bayanus and three between S. cerevisiae and S. kudriavzevii. Furthermore, this microarray design allowed the detection of multiple introgressed S. paradoxus DNA fragments in the genomes of three different S. cerevisiae isolates. These results show the power of multispecies-based microarrays as taxonomic tools for the identification of species and interspecies hybrids, and their ability to provide a more detailed characterization of interspecies hybrids and recombinants.

Full Text

Duke Authors

Cited Authors

  • Muller, LAH; McCusker, JH

Published Date

  • February 2009

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 143 - 152

PubMed ID

  • 19054123

Pubmed Central ID

  • 19054123

Electronic International Standard Serial Number (EISSN)

  • 1567-1364

Digital Object Identifier (DOI)

  • 10.1111/j.1567-1364.2008.00464.x

Language

  • eng

Conference Location

  • England