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Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo.

Publication ,  Journal Article
Sprengel, R; Suchanek, B; Amico, C; Brusa, R; Burnashev, N; Rozov, A; Hvalby, O; Jensen, V; Paulsen, O; Andersen, P; Kim, JJ; Thompson, RF ...
Published in: Cell
January 23, 1998

NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity of central excitatory synapses. We show here that gene-targeted mice expressing NMDA receptors without the large intracellular C-terminal domain of any one of three NR2 subunits phenotypically resemble mice made deficient in that particular subunit. Mice expressing the NR2B subunit in a C-terminally truncated form (NR2B(deltaC/deltaC) mice) die perinatally. NR2A(deltaC/deltaC) mice are viable but exhibit impaired synaptic plasticity and contextual memory. These and NR2C(deltaC/deltaC) mice display deficits in motor coordination. C-terminal truncation of NR2 subunits does not interfere with the formation of gateable receptor channels that can be synaptically activated. Thus, the phenotypes of our mutants appear to reflect defective intracellular signaling.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

January 23, 1998

Volume

92

Issue

2

Start / End Page

279 / 289

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Sequence Deletion
  • Receptors, N-Methyl-D-Aspartate
  • Postural Balance
  • Nerve Tissue Proteins
  • Motor Skills
  • Molecular Sequence Data
  • Mice, Knockout
  • Mice
  • Male
 

Citation

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Sprengel, R., Suchanek, B., Amico, C., Brusa, R., Burnashev, N., Rozov, A., … Seeburg, P. H. (1998). Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo. Cell, 92(2), 279–289. https://doi.org/10.1016/s0092-8674(00)80921-6
Sprengel, R., B. Suchanek, C. Amico, R. Brusa, N. Burnashev, A. Rozov, O. Hvalby, et al. “Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo.Cell 92, no. 2 (January 23, 1998): 279–89. https://doi.org/10.1016/s0092-8674(00)80921-6.
Sprengel R, Suchanek B, Amico C, Brusa R, Burnashev N, Rozov A, et al. Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo. Cell. 1998 Jan 23;92(2):279–89.
Sprengel, R., et al. “Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo.Cell, vol. 92, no. 2, Jan. 1998, pp. 279–89. Pubmed, doi:10.1016/s0092-8674(00)80921-6.
Sprengel R, Suchanek B, Amico C, Brusa R, Burnashev N, Rozov A, Hvalby O, Jensen V, Paulsen O, Andersen P, Kim JJ, Thompson RF, Sun W, Webster LC, Grant SG, Eilers J, Konnerth A, Li J, McNamara JO, Seeburg PH. Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo. Cell. 1998 Jan 23;92(2):279–289.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

January 23, 1998

Volume

92

Issue

2

Start / End Page

279 / 289

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Sequence Deletion
  • Receptors, N-Methyl-D-Aspartate
  • Postural Balance
  • Nerve Tissue Proteins
  • Motor Skills
  • Molecular Sequence Data
  • Mice, Knockout
  • Mice
  • Male