Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo.

Published

Journal Article

NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity of central excitatory synapses. We show here that gene-targeted mice expressing NMDA receptors without the large intracellular C-terminal domain of any one of three NR2 subunits phenotypically resemble mice made deficient in that particular subunit. Mice expressing the NR2B subunit in a C-terminally truncated form (NR2B(deltaC/deltaC) mice) die perinatally. NR2A(deltaC/deltaC) mice are viable but exhibit impaired synaptic plasticity and contextual memory. These and NR2C(deltaC/deltaC) mice display deficits in motor coordination. C-terminal truncation of NR2 subunits does not interfere with the formation of gateable receptor channels that can be synaptically activated. Thus, the phenotypes of our mutants appear to reflect defective intracellular signaling.

Full Text

Duke Authors

Cited Authors

  • Sprengel, R; Suchanek, B; Amico, C; Brusa, R; Burnashev, N; Rozov, A; Hvalby, O; Jensen, V; Paulsen, O; Andersen, P; Kim, JJ; Thompson, RF; Sun, W; Webster, LC; Grant, SG; Eilers, J; Konnerth, A; Li, J; McNamara, JO; Seeburg, PH

Published Date

  • January 1998

Published In

Volume / Issue

  • 92 / 2

Start / End Page

  • 279 - 289

PubMed ID

  • 9458051

Pubmed Central ID

  • 9458051

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)80921-6

Language

  • eng