Multi-analyte profiling of ten cytokines in South African HIV-infected patients with Immune Reconstitution Inflammatory Syndrome (IRIS).

Published online

Journal Article

BACKGROUND: Immune reconstitution inflammatory syndrome (IRIS) is an important complication of HAART in sub-Saharan Africa, where opportunistic infections (OIs) including mycobacteria and cryptococcus are common. The immune system's role in HIV infected patients is complex with cytokine expression strongly influencing HIV infection and replication. METHODS: We determined the expression patterns of 10 cytokines by Luminex multi-analyte profiling in 17 IRIS nested case-control pairs participating in a prospective South African cohort initiating anti-retroviral therapy. RESULTS: Interferon-gamma (IFN-γ) expression was significantly elevated in IRIS cases compared to controls (median 9.88 pg/ml versus 2.68 pg/ml, respectively, P = 0.0057), while other cytokines displayed non-significant differences in expression. Significant correlation was observed between IL-6, IL-10, and IFN-γ expression in the IRIS patients. CONCLUSIONS: Significantly increased expression levels of IFN-γ suggest that this cytokine possibly plays a role in IRIS pathology and is a potential diagnostic marker.

Full Text

Duke Authors

Cited Authors

  • Worsley, CM; Suchard, MS; Stevens, WS; Van Rie, A; Murdoch, DM

Published Date

  • October 7, 2010

Published In

Volume / Issue

  • 7 /

Start / End Page

  • 36 -

PubMed ID

  • 20929543

Pubmed Central ID

  • 20929543

Electronic International Standard Serial Number (EISSN)

  • 1742-6405

Digital Object Identifier (DOI)

  • 10.1186/1742-6405-7-36

Language

  • eng

Conference Location

  • England