Expression profiling of a transformed thymocyte cell line undergoing maturation in vitro identifies multiple genes involved in positive selection.

Published

Journal Article

Biochemical and genetic studies of thymocyte maturation would be facilitated by the development of cultured cell lines that reflect stages of positive selection. We have derived a CD4(+)CD8(+)TCR(+) T-lymphoid cell line (M20) from a murine thymic tumor induced by a retrovirus carrying the v-myc oncogene (M-MuLV(myc)). M20 subclones undergo several aspects of positive selection in response to co-culture with a thymic stromal cell line (St3), including down-regulation of CD4 and CD8, and up-regulation of CD5 and TCR. M20 possesses a functional TCR complex, and ligation of this complex produces changes similar to co-culture with St3 stroma. Expression profiling of M20 cells in this system identified 23 genes previously shown to be important in thymocyte maturation, as well as several novel candidate genes. This system provides a new model to elucidate the molecular mechanisms of thymocyte maturation and TCR-mediated cell signaling in double-positive thymocytes.

Full Text

Duke Authors

Cited Authors

  • Tiong Ong, S; Ly, C; Nguyen, M; Kay Brightman, B; Fan, H

Published Date

  • January 2003

Published In

Volume / Issue

  • 221 / 1

Start / End Page

  • 64 - 79

PubMed ID

  • 12742383

Pubmed Central ID

  • 12742383

International Standard Serial Number (ISSN)

  • 0008-8749

Language

  • eng

Conference Location

  • Netherlands