Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae.

Journal Article (Journal Article)

Yeast strains with mutations in both TEL1 and MEC1 have short telomeres and elevated rates of chromosome deletions. By using a PCR assay, we demonstrate that mec1 tel1 strains also have telomere-telomere fusions (T-TFs). T-TFs require Lig4p (a ligase required for nonhomologous end-joining DNA repair). The highest rates of T-TFs are found in strains with combination of mutations that affect telomere length and DNA damage checkpoints (mec1 tel1, mec3 tel1, mre11 mec1, and ddc1 tel1 strains). Examining many mutant genotypes, we find good agreement between the level of T-TFs and the rate of chromosomal deletions. In addition, if telomeres are elongated in a mec1 tel1 strain, we eliminate T-TFs and reduce the deletion rate. The correlation between the level of T-TFs and the rate of deletions argues that many of these deletions reflect a cycle of T-TF formation (resulting in dicentric chromosomes), followed by chromosome breakage.

Full Text

Duke Authors

Cited Authors

  • Mieczkowski, PA; Mieczkowska, JO; Dominska, M; Petes, TD

Published Date

  • September 16, 2003

Published In

Volume / Issue

  • 100 / 19

Start / End Page

  • 10854 - 10859

PubMed ID

  • 12963812

Pubmed Central ID

  • PMC196892

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1934561100


  • eng

Conference Location

  • United States