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Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae.

Publication ,  Journal Article
Mieczkowski, PA; Mieczkowska, JO; Dominska, M; Petes, TD
Published in: Proc Natl Acad Sci U S A
September 16, 2003

Yeast strains with mutations in both TEL1 and MEC1 have short telomeres and elevated rates of chromosome deletions. By using a PCR assay, we demonstrate that mec1 tel1 strains also have telomere-telomere fusions (T-TFs). T-TFs require Lig4p (a ligase required for nonhomologous end-joining DNA repair). The highest rates of T-TFs are found in strains with combination of mutations that affect telomere length and DNA damage checkpoints (mec1 tel1, mec3 tel1, mre11 mec1, and ddc1 tel1 strains). Examining many mutant genotypes, we find good agreement between the level of T-TFs and the rate of chromosomal deletions. In addition, if telomeres are elongated in a mec1 tel1 strain, we eliminate T-TFs and reduce the deletion rate. The correlation between the level of T-TFs and the rate of deletions argues that many of these deletions reflect a cycle of T-TF formation (resulting in dicentric chromosomes), followed by chromosome breakage.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 16, 2003

Volume

100

Issue

19

Start / End Page

10854 / 10859

Location

United States

Related Subject Headings

  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • DNA, Fungal
  • Base Sequence
 

Citation

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ICMJE
MLA
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Mieczkowski, P. A., Mieczkowska, J. O., Dominska, M., & Petes, T. D. (2003). Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae. Proc Natl Acad Sci U S A, 100(19), 10854–10859. https://doi.org/10.1073/pnas.1934561100
Mieczkowski, Piotr A., Joanna O. Mieczkowska, Margaret Dominska, and Thomas D. Petes. “Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae.Proc Natl Acad Sci U S A 100, no. 19 (September 16, 2003): 10854–59. https://doi.org/10.1073/pnas.1934561100.
Mieczkowski PA, Mieczkowska JO, Dominska M, Petes TD. Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10854–9.
Mieczkowski, Piotr A., et al. “Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae.Proc Natl Acad Sci U S A, vol. 100, no. 19, Sept. 2003, pp. 10854–59. Pubmed, doi:10.1073/pnas.1934561100.
Mieczkowski PA, Mieczkowska JO, Dominska M, Petes TD. Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10854–10859.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 16, 2003

Volume

100

Issue

19

Start / End Page

10854 / 10859

Location

United States

Related Subject Headings

  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • DNA, Fungal
  • Base Sequence