Systems biology of vaccination for seasonal influenza in humans.

Published online

Journal Article

Here we have used a systems biology approach to study innate and adaptive responses to vaccination against influenza in humans during three consecutive influenza seasons. We studied healthy adults vaccinated with trivalent inactivated influenza vaccine (TIV) or live attenuated influenza vaccine (LAIV). TIV induced higher antibody titers and more plasmablasts than LAIV did. In subjects vaccinated with TIV, early molecular signatures correlated with and could be used to accurately predict later antibody titers in two independent trials. Notably, expression of the kinase CaMKIV at day 3 was inversely correlated with later antibody titers. Vaccination of CaMKIV-deficient mice with TIV induced enhanced antigen-specific antibody titers, which demonstrated an unappreciated role for CaMKIV in the regulation of antibody responses. Thus, systems approaches can be used to predict immunogenicity and provide new mechanistic insights about vaccines.

Full Text

Duke Authors

Cited Authors

  • Nakaya, HI; Wrammert, J; Lee, EK; Racioppi, L; Marie-Kunze, S; Haining, WN; Means, AR; Kasturi, SP; Khan, N; Li, G-M; McCausland, M; Kanchan, V; Kokko, KE; Li, S; Elbein, R; Mehta, AK; Aderem, A; Subbarao, K; Ahmed, R; Pulendran, B

Published Date

  • July 10, 2011

Published In

Volume / Issue

  • 12 / 8

Start / End Page

  • 786 - 795

PubMed ID

  • 21743478

Pubmed Central ID

  • 21743478

Electronic International Standard Serial Number (EISSN)

  • 1529-2916

Digital Object Identifier (DOI)

  • 10.1038/ni.2067

Language

  • eng

Conference Location

  • United States