Molecular structures of crossover and noncrossover intermediates during gap repair in yeast: implications for recombination.

Journal Article (Journal Article)

The molecular structures of crossover (CO) and noncrossover (NCO) intermediates were determined by sequencing the products formed when a gapped plasmid was repaired using a diverged chromosomal template. Analyses were done in the absence of mismatch repair (MMR) to allow efficient detection of strand-transfer intermediates, and the results reveal striking differences in the extents and locations of heteroduplex DNA (hDNA) in NCO versus CO products. These data indicate that most NCOs are produced by synthesis-dependent strand annealing rather than by a canonical double-strand break repair pathway and that resolution of Holliday junctions formed as part of the latter pathway is highly constrained to generate CO products. We suggest a model in which the length of hDNA formed by the initiating strand invasion event determines susceptibility of the resulting intermediate to antirecombination and ultimately whether a CO- or a NCO-producing pathway is followed.

Full Text

Duke Authors

Cited Authors

  • Mitchel, K; Zhang, H; Welz-Voegele, C; Jinks-Robertson, S

Published Date

  • April 23, 2010

Published In

Volume / Issue

  • 38 / 2

Start / End Page

  • 211 - 222

PubMed ID

  • 20417600

Pubmed Central ID

  • PMC2865147

Electronic International Standard Serial Number (EISSN)

  • 1097-4164

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2010.02.028


  • eng

Conference Location

  • United States