Overview
My research focuses on the regulation of genetic stability and primarily uses budding yeast (Saccharomyces cerevisiae) as a model genetic system. The two primary research goals in the budding yeast system are (1) defining molecular structures and mechanisms of mitotic recombination intermediates and (2) understanding how and why transcription destabilizes the underlying DNA template. We also have initiated studies of mutagenesis in the pathogenic fungus Cryptococcus neoformans. We have …
Current Appointments & Affiliations
James B. Duke Distinguished Professor Emerita
·
2023 - Present
Molecular Genetics and Microbiology,
Basic Science Departments
Professor Emeritus of Molecular Genetics and Microbiology
·
2023 - Present
Molecular Genetics and Microbiology,
Basic Science Departments
Member of the Duke Cancer Institute
·
2006 - Present
Duke Cancer Institute,
Institutes and Centers
Recent Publications
Spontaneous deamination of cytosine to uracil is biased to the non-transcribed DNA strand in yeast.
Journal Article DNA Repair (Amst) · June 2023 Transcription in Saccharomyces cerevisiae is associated with elevated mutation and this partially reflects enhanced damage of the corresponding DNA. Spontaneous deamination of cytosine to uracil leads to CG>TA mutations that provide a strand-specific read- ... Full text Open Access Link to item CiteGenetic control of the error-prone repair of a chromosomal double-strand break with 5' overhangs in yeast.
Preprint · May 4, 2023 Full text Link to item CiteGenome-wide analysis of heat stress-stimulated transposon mobility in the human fungal pathogen Cryptococcus deneoformans.
Journal Article Proc Natl Acad Sci U S A · January 24, 2023 We recently reported transposon mutagenesis as a significant driver of spontaneous mutations in the human fungal pathogen Cryptococcus deneoformans during murine infection. Mutations caused by transposable element (TE) insertion into reporter genes were dr ... Full text Link to item CiteRecent Grants
The role of transcription factor proteins in mutagenesis at their binding sites
ResearchCollaborator · Awarded by National Institutes of Health · 2020 - 2025PacBio Sequel IIe for GCB Sequencing Core
EquipmentMajor User · Awarded by National Institutes of Health · 2023 - 2024Regulation of mitotic genome stability in yeast.
ResearchPrincipal Investigator · Awarded by National Institutes of Health · 2016 - 2023View All Grants
Education, Training & Certifications
University of Wisconsin, Madison ·
1983
Ph.D.