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Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole.

Publication ,  Journal Article
Espinel-Ingroff, A; Fothergill, A; Ghannoum, M; Manavathu, E; Ostrosky-Zeichner, L; Pfaller, M; Rinaldi, M; Schell, W; Walsh, T
Published in: J Clin Microbiol
October 2005

Although standard conditions are available for testing the susceptibilities of filamentous fungi to antifungal agents by the Clinical and Laboratory Standards Institute (CLSI; formerly National Committee for Clinical Laboratory Standards) broth microdilution assay, quality control (QC) MIC limits have not been established for any mold-agent combination. This multicenter (eight-center) study documented the reproducibility of tests for one isolate of Paecilomyces variotii ATCC MYA-3630 and 11 other mold isolates (three isolates of Aspergillus fumigatus; two isolates of A. terreus; one isolate each of A. flavus, A. nidulans, Fusarium moniliforme, and F. solani; and two isolates of Scedosporium apiospermum) by the CLSI reference broth microdilution method (M 38-A document). Control limits (amphotericin B, 1 to 4 microg/ml; itraconazole, 0.06 to 0.5 microg/ml; posaconazole, 0.03 to 0.25 microg/ml; voriconazole, 0.015 to 0.12 microg/ml) for the selected QC P. variotii ATCC MYA-3630 were established by the analysis of replicate MIC results. Reference isolates and corresponding MIC ranges were also established for 6 of the 12 molds evaluated. MIC limits were not proposed for the other five molds tested due to low testing reproducibility for these isolates.

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Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

October 2005

Volume

43

Issue

10

Start / End Page

5243 / 5246

Location

United States

Related Subject Headings

  • Voriconazole
  • Triazoles
  • Quality Control
  • Pyrimidines
  • Paecilomyces
  • Microbiology
  • Microbial Sensitivity Tests
  • Itraconazole
  • Humans
  • Fungi
 

Citation

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Espinel-Ingroff, A., Fothergill, A., Ghannoum, M., Manavathu, E., Ostrosky-Zeichner, L., Pfaller, M., … Walsh, T. (2005). Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J Clin Microbiol, 43(10), 5243–5246. https://doi.org/10.1128/JCM.43.10.5243-5246.2005
Espinel-Ingroff, A., A. Fothergill, M. Ghannoum, E. Manavathu, L. Ostrosky-Zeichner, M. Pfaller, M. Rinaldi, W. Schell, and T. Walsh. “Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole.J Clin Microbiol 43, no. 10 (October 2005): 5243–46. https://doi.org/10.1128/JCM.43.10.5243-5246.2005.
Espinel-Ingroff A, Fothergill A, Ghannoum M, Manavathu E, Ostrosky-Zeichner L, Pfaller M, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J Clin Microbiol. 2005 Oct;43(10):5243–6.
Espinel-Ingroff, A., et al. “Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole.J Clin Microbiol, vol. 43, no. 10, Oct. 2005, pp. 5243–46. Pubmed, doi:10.1128/JCM.43.10.5243-5246.2005.
Espinel-Ingroff A, Fothergill A, Ghannoum M, Manavathu E, Ostrosky-Zeichner L, Pfaller M, Rinaldi M, Schell W, Walsh T. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J Clin Microbiol. 2005 Oct;43(10):5243–5246.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

October 2005

Volume

43

Issue

10

Start / End Page

5243 / 5246

Location

United States

Related Subject Headings

  • Voriconazole
  • Triazoles
  • Quality Control
  • Pyrimidines
  • Paecilomyces
  • Microbiology
  • Microbial Sensitivity Tests
  • Itraconazole
  • Humans
  • Fungi