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Silver nanoparticles compromise neurodevelopment in PC12 cells: critical contributions of silver ion, particle size, coating, and composition.

Publication ,  Journal Article
Powers, CM; Badireddy, AR; Ryde, IT; Seidler, FJ; Slotkin, TA
Published in: Environ Health Perspect
January 2011

BACKGROUND: Silver exposures are rising because of the increased use of silver nanoparticles (AgNPs) in consumer products. The monovalent silver ion (Ag+) impairs neurodevelopment in PC12 cells and zebrafish. OBJECTIVES AND METHODS: We compared the effects of AgNPs with Ag+ in PC12 cells for neurodevelopmental end points including cell replication, oxidative stress, cell viability, and differentiation. First, we compared citrate-coated AgNPs (AgNP-Cs) with Ag+, and then we assessed the roles of particle size, coating, and composition by comparing AgNP-C with two different sizes of polyvinylpyrrolidone-coated AgNPs (AgNP-PVPs) or silica nanoparticles. RESULTS: In undifferentiated cells, AgNP-C impaired DNA synthesis, but to a lesser extent than an equivalent nominal concentration of Ag+, whereas AgNP-C and Ag+ were equally effective against protein synthesis; there was little or no oxidative stress or loss of viability due to AgNP-C. In contrast, in differentiating cells, AgNP-C evoked robust oxidative stress and impaired differentiation into the acetylcholine phenotype. Although the effects of AgNP-PVP showed similarities to those of AgNP-C, we also found significant differences in potencies and differentiation outcomes that depended both on particle size and coating. None of the effects reflected simple physical attributes of nanoparticles, separate from composition or coating, as equivalent concentrations of silica nanoparticles had no detectable effects. CONCLUSIONS: AgNP exposure impairs neurodevelopment in PC12 cells. Further, AgNP effects are distinct from those of Ag+ alone and depend on size and coating, indicating that AgNP effects are not due simply to the release of Ag+ into the surrounding environment.

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Published In

Environ Health Perspect

DOI

EISSN

1552-9924

Publication Date

January 2011

Volume

119

Issue

1

Start / End Page

37 / 44

Location

United States

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Silver
  • Rats
  • Particle Size
  • PC12 Cells
  • Oxidative Stress
  • Metal Nanoparticles
  • Growth and Development
  • Central Nervous System
 

Citation

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Powers, C. M., Badireddy, A. R., Ryde, I. T., Seidler, F. J., & Slotkin, T. A. (2011). Silver nanoparticles compromise neurodevelopment in PC12 cells: critical contributions of silver ion, particle size, coating, and composition. Environ Health Perspect, 119(1), 37–44. https://doi.org/10.1289/ehp.1002337
Powers, Christina M., Appala R. Badireddy, Ian T. Ryde, Frederic J. Seidler, and Theodore A. Slotkin. “Silver nanoparticles compromise neurodevelopment in PC12 cells: critical contributions of silver ion, particle size, coating, and composition.Environ Health Perspect 119, no. 1 (January 2011): 37–44. https://doi.org/10.1289/ehp.1002337.
Powers CM, Badireddy AR, Ryde IT, Seidler FJ, Slotkin TA. Silver nanoparticles compromise neurodevelopment in PC12 cells: critical contributions of silver ion, particle size, coating, and composition. Environ Health Perspect. 2011 Jan;119(1):37–44.
Powers, Christina M., et al. “Silver nanoparticles compromise neurodevelopment in PC12 cells: critical contributions of silver ion, particle size, coating, and composition.Environ Health Perspect, vol. 119, no. 1, Jan. 2011, pp. 37–44. Pubmed, doi:10.1289/ehp.1002337.
Powers CM, Badireddy AR, Ryde IT, Seidler FJ, Slotkin TA. Silver nanoparticles compromise neurodevelopment in PC12 cells: critical contributions of silver ion, particle size, coating, and composition. Environ Health Perspect. 2011 Jan;119(1):37–44.

Published In

Environ Health Perspect

DOI

EISSN

1552-9924

Publication Date

January 2011

Volume

119

Issue

1

Start / End Page

37 / 44

Location

United States

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Silver
  • Rats
  • Particle Size
  • PC12 Cells
  • Oxidative Stress
  • Metal Nanoparticles
  • Growth and Development
  • Central Nervous System