Dissection of a type I interferon pathway in controlling bacterial intracellular infection in mice.
Published
Journal Article
Defence mechanisms against intracellular bacterial pathogens are incompletely understood. Our study characterizes a type I IFN-dependent cell-autonomous defence pathway directed against Legionella pneumophila, an intracellular model organism and frequent cause of pneumonia. We show that macrophages infected with L. pneumophila produced IFNβ in a STING- and IRF3- dependent manner. Paracrine type I IFNs stimulated upregulation of IFN-stimulated genes and a cell-autonomous defence pathway acting on replicating and non-replicating Legionella within their specialized vacuole. Our infection experiments in mice lacking receptors for type I and/or II IFNs show that type I IFNs contribute to expression of IFN-stimulated genes and to bacterial clearance as well as resistance in L. pneumophila pneumonia in addition to type II IFN. Overall, our study shows that paracrine type I IFNs mediate defence against L. pneumophila, and demonstrates a protective role of type I IFNs in in vivo infections with intracellular bacteria.
Full Text
Duke Authors
Cited Authors
- Lippmann, J; Müller, HC; Naujoks, J; Tabeling, C; Shin, S; Witzenrath, M; Hellwig, K; Kirschning, CJ; Taylor, GA; Barchet, W; Bauer, S; Suttorp, N; Roy, CR; Opitz, B
Published Date
- November 2011
Published In
Volume / Issue
- 13 / 11
Start / End Page
- 1668 - 1682
PubMed ID
- 21790939
Pubmed Central ID
- 21790939
Electronic International Standard Serial Number (EISSN)
- 1462-5822
Digital Object Identifier (DOI)
- 10.1111/j.1462-5822.2011.01646.x
Language
- eng
Conference Location
- England