Met and hepatocyte growth factor/scatter factor expression in human gliomas.

Journal Article (Journal Article)

Using double immunofluorescence staining and quantitative confocal laser scan microscopy, we show that the intensity of hepatocyte growth factor/scatter factor (HGF/SF) and Met staining in human primary brain tumors increases with the grade of malignancy and is prevalent in both the infiltrating tumor cells and endothelial hyperplastic areas. HGF/SF and Met also are expressed in vitro in glioblastoma multiforme cell lines as well as in normal human astrocyte (NHA) cells. Moreover, HGF/SF stimulates tyrosine phosphorylation of Met in both glioma cell lines and NHA cells, but only the glioma cell lines proliferate and become motile and invasive in response to HGF/SF, whereas the NHA cells are nonresponsive. These results implicate autocrine/paracrine Met-HGF/SF signaling in glioma tumorigenesis and suggest that HGF/SF signaling through Met is negatively regulated in NHA cells.

Full Text

Duke Authors

Cited Authors

  • Koochekpour, S; Jeffers, M; Rulong, S; Taylor, G; Klineberg, E; Hudson, EA; Resau, JH; Vande Woude, GF

Published Date

  • December 1, 1997

Published In

Volume / Issue

  • 57 / 23

Start / End Page

  • 5391 - 5398

PubMed ID

  • 9393765

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States