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Met and hepatocyte growth factor/scatter factor expression in human gliomas.

Publication ,  Journal Article
Koochekpour, S; Jeffers, M; Rulong, S; Taylor, G; Klineberg, E; Hudson, EA; Resau, JH; Vande Woude, GF
Published in: Cancer Res
December 1, 1997

Using double immunofluorescence staining and quantitative confocal laser scan microscopy, we show that the intensity of hepatocyte growth factor/scatter factor (HGF/SF) and Met staining in human primary brain tumors increases with the grade of malignancy and is prevalent in both the infiltrating tumor cells and endothelial hyperplastic areas. HGF/SF and Met also are expressed in vitro in glioblastoma multiforme cell lines as well as in normal human astrocyte (NHA) cells. Moreover, HGF/SF stimulates tyrosine phosphorylation of Met in both glioma cell lines and NHA cells, but only the glioma cell lines proliferate and become motile and invasive in response to HGF/SF, whereas the NHA cells are nonresponsive. These results implicate autocrine/paracrine Met-HGF/SF signaling in glioma tumorigenesis and suggest that HGF/SF signaling through Met is negatively regulated in NHA cells.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

December 1, 1997

Volume

57

Issue

23

Start / End Page

5391 / 5398

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Recombinant Proteins
  • Proto-Oncogene Proteins c-met
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Morphogenesis
  • Mice
  • Humans
  • Hepatocyte Growth Factor
 

Citation

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Koochekpour, S., Jeffers, M., Rulong, S., Taylor, G., Klineberg, E., Hudson, E. A., … Vande Woude, G. F. (1997). Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res, 57(23), 5391–5398.
Koochekpour, S., M. Jeffers, S. Rulong, G. Taylor, E. Klineberg, E. A. Hudson, J. H. Resau, and G. F. Vande Woude. “Met and hepatocyte growth factor/scatter factor expression in human gliomas.Cancer Res 57, no. 23 (December 1, 1997): 5391–98.
Koochekpour S, Jeffers M, Rulong S, Taylor G, Klineberg E, Hudson EA, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 1997 Dec 1;57(23):5391–8.
Koochekpour, S., et al. “Met and hepatocyte growth factor/scatter factor expression in human gliomas.Cancer Res, vol. 57, no. 23, Dec. 1997, pp. 5391–98.
Koochekpour S, Jeffers M, Rulong S, Taylor G, Klineberg E, Hudson EA, Resau JH, Vande Woude GF. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 1997 Dec 1;57(23):5391–5398.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

December 1, 1997

Volume

57

Issue

23

Start / End Page

5391 / 5398

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Recombinant Proteins
  • Proto-Oncogene Proteins c-met
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Morphogenesis
  • Mice
  • Humans
  • Hepatocyte Growth Factor