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De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex.

Publication ,  Journal Article
Korendovych, IV; Senes, A; Kim, YH; Lear, JD; Fry, HC; Therien, MJ; Blasie, JK; Walker, FA; Degrado, WF
Published in: Journal of the American Chemical Society
November 2010

The de novo design of membrane proteins remains difficult despite recent advances in understanding the factors that drive membrane protein folding and association. We have designed a membrane protein PRIME (PoRphyrins In MEmbrane) that positions two non-natural iron diphenylporphyrins (Fe(III)DPP's) sufficiently close to provide a multicentered pathway for transmembrane electron transfer. Computational methods previously used for the design of multiporphyrin water-soluble helical proteins were extended to this membrane target. Four helices were arranged in a D(2)-symmetrical bundle to bind two Fe(II/III) diphenylporphyrins in a bis-His geometry further stabilized by second-shell hydrogen bonds. UV-vis absorbance, CD spectroscopy, analytical ultracentrifugation, redox potentiometry, and EPR demonstrate that PRIME binds the cofactor with high affinity and specificity in the expected geometry.

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Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

November 2010

Volume

132

Issue

44

Start / End Page

15516 / 15518

Related Subject Headings

  • Protein Folding
  • Protein Binding
  • Porphyrins
  • Multiprotein Complexes
  • Models, Molecular
  • Membrane Proteins
  • General Chemistry
  • Circular Dichroism
  • 40 Engineering
  • 34 Chemical sciences
 

Citation

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Korendovych, I. V., Senes, A., Kim, Y. H., Lear, J. D., Fry, H. C., Therien, M. J., … Degrado, W. F. (2010). De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex. Journal of the American Chemical Society, 132(44), 15516–15518. https://doi.org/10.1021/ja107487b
Korendovych, Ivan V., Alessandro Senes, Yong Ho Kim, James D. Lear, H Christopher Fry, Michael J. Therien, J Kent Blasie, F Ann Walker, and William F. Degrado. “De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex.Journal of the American Chemical Society 132, no. 44 (November 2010): 15516–18. https://doi.org/10.1021/ja107487b.
Korendovych IV, Senes A, Kim YH, Lear JD, Fry HC, Therien MJ, et al. De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex. Journal of the American Chemical Society. 2010 Nov;132(44):15516–8.
Korendovych, Ivan V., et al. “De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex.Journal of the American Chemical Society, vol. 132, no. 44, Nov. 2010, pp. 15516–18. Epmc, doi:10.1021/ja107487b.
Korendovych IV, Senes A, Kim YH, Lear JD, Fry HC, Therien MJ, Blasie JK, Walker FA, Degrado WF. De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex. Journal of the American Chemical Society. 2010 Nov;132(44):15516–15518.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

November 2010

Volume

132

Issue

44

Start / End Page

15516 / 15518

Related Subject Headings

  • Protein Folding
  • Protein Binding
  • Porphyrins
  • Multiprotein Complexes
  • Models, Molecular
  • Membrane Proteins
  • General Chemistry
  • Circular Dichroism
  • 40 Engineering
  • 34 Chemical sciences