In vivo fluorescence imaging: a personal perspective.
Published
Journal Article (Review)
In vivo fluorescence imaging with near-infrared (NIR) light holds enormous potential for a wide variety of molecular diagnostic and therapeutic applications. Because of its quantitative sensitivity, inherent biological safety, and relative ease of use (i.e., with respect to cost, time, mobility, and its familiarity to a diverse population of investigators), fluorescence-based imaging techniques are being increasingly utilized in small-animal research. Moreover, there is substantial interest in the translation of novel optical techniques into the clinic, where they will prospectively aid in noninvasive and quantitative screening, disease diagnosis, and post-treatment monitoring of patients. Effective deep-tissue fluorescence imaging requires the application of exogenous NIR-emissive contrast agents. Currently, available probes fall into two major categories: organic and inorganic NIR fluorophores (NIRFs). In the studies reviewed herein, we utilized polymersomes (50 nm to 50 microm diameter polymer vesicles) for the incorporation and delivery of large numbers of highly emissive oligo (porphyrin)-based, organic NIRFs.
Full Text
Duke Authors
Cited Authors
- Ghoroghchian, PP; Therien, MJ; Hammer, DA
Published Date
- March 2009
Published In
Volume / Issue
- 1 / 2
Start / End Page
- 156 - 167
PubMed ID
- 20049787
Pubmed Central ID
- 20049787
Electronic International Standard Serial Number (EISSN)
- 1939-0041
International Standard Serial Number (ISSN)
- 1939-5116
Digital Object Identifier (DOI)
- 10.1002/wnan.7
Language
- eng