A multidisciplinary approach to probing enthalpy-entropy compensation and the interfacial mobility model.
In recent years, interfacial mobility has gained popularity as a model with which to rationalize both affinity in ligand binding and the often observed phenomenon of enthalpy-entropy compensation. While protein contraction and reduced mobility, as demonstrated by computational and NMR techniques respectively, have been correlated to entropies of binding for a variety of systems, to our knowledge, Raman difference spectroscopy has never been included in these analyses. Here, nonresonance Raman difference spectroscopy, isothermal titration calorimetry, and X-ray crystallography were utilized to correlate protein contraction, as demonstrated by an increase in protein interior packing and decreased residual protein movement, with trends of enthalpy-entropy compensation. These results are in accord with the interfacial mobility model and lend additional credence to this view of protein activity.
Wilfong, EM; Kogiso, Y; Muthukrishnan, S; Kowatz, T; Du, Y; Bowie, A; Naismith, JH; Hadad, CM; Toone, EJ; Gustafson, TL
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