Skip to main content
Journal cover image

Interleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells.

Publication ,  Journal Article
Goldgaber, D; Harris, HW; Hla, T; Maciag, T; Donnelly, RJ; Jacobsen, JS; Vitek, MP; Gajdusek, DC
Published in: Proc Natl Acad Sci U S A
October 1989

We have analyzed the modulation of amyloid beta-protein precursor (APP) gene expression in human umbilical vein endothelial cells (HUVEC). The level of the APP mRNA transcripts increased as HUVEC reached confluency. In confluent culture the half-life of the APP mRNA was 4 hr. Treatment of the cells with human-recombinant interleukin 1 (IL-1), phorbol 12-myristate 13-acetate, or heparin-binding growth factor 1 enhanced the expression of APP gene in these cells, but calcium ionophore A23187 and dexamethasone did not. The protein kinase C inhibitor 1-(isoquinolinsulfonyl)-2-methylpiperazine (H7) inhibited IL-1-mediated increase of the level of APP transcripts. To map IL-1-responsive elements of the APP promoter, truncated portions of the APP promoter were fused to the human growth hormone reporter gene. The recombinant plasmids were transfected into mouse neuroblastoma cells, and the cell medium was assayed for the human growth hormone. A 180-base-pair region of the APP promoter located between position -485 and -305 upstream from the transcription start site was necessary for IL-1-mediated induction of the reporter gene. This region contains the upstream transcription factor AP-1 binding site. These results suggest that IL-1 upregulates APP gene expression in HUVEC through a pathway mediated by protein kinase C, utilizing the upstream AP-1 binding site of the APP promoter.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1989

Volume

86

Issue

19

Start / End Page

7606 / 7610

Location

United States

Related Subject Headings

  • Transfection
  • Recombinant Proteins
  • RNA, Messenger
  • Protein Precursors
  • Protease Inhibitors
  • Promoter Regions, Genetic
  • Plasmids
  • Interleukin-1
  • Humans
  • Endothelium, Vascular
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Goldgaber, D., Harris, H. W., Hla, T., Maciag, T., Donnelly, R. J., Jacobsen, J. S., … Gajdusek, D. C. (1989). Interleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells. Proc Natl Acad Sci U S A, 86(19), 7606–7610. https://doi.org/10.1073/pnas.86.19.7606
Goldgaber, D., H. W. Harris, T. Hla, T. Maciag, R. J. Donnelly, J. S. Jacobsen, M. P. Vitek, and D. C. Gajdusek. “Interleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells.Proc Natl Acad Sci U S A 86, no. 19 (October 1989): 7606–10. https://doi.org/10.1073/pnas.86.19.7606.
Goldgaber D, Harris HW, Hla T, Maciag T, Donnelly RJ, Jacobsen JS, et al. Interleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7606–10.
Goldgaber, D., et al. “Interleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells.Proc Natl Acad Sci U S A, vol. 86, no. 19, Oct. 1989, pp. 7606–10. Pubmed, doi:10.1073/pnas.86.19.7606.
Goldgaber D, Harris HW, Hla T, Maciag T, Donnelly RJ, Jacobsen JS, Vitek MP, Gajdusek DC. Interleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7606–7610.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1989

Volume

86

Issue

19

Start / End Page

7606 / 7610

Location

United States

Related Subject Headings

  • Transfection
  • Recombinant Proteins
  • RNA, Messenger
  • Protein Precursors
  • Protease Inhibitors
  • Promoter Regions, Genetic
  • Plasmids
  • Interleukin-1
  • Humans
  • Endothelium, Vascular