Nonlinear absorption microscopy.

Journal Article (Review;Journal Article)

For the past two decades, nonlinear microscopy has been developed to overcome the scattering problem in thick tissue imaging. Owing to its increased imaging depth and high spatial resolution, nonlinear microscopy becomes the first choice for imaging living tissues. The use of nonlinear optical effects not only facilitates the signal originating from an extremely small volume defined by light focusing but also provides novel contrast mechanisms with molecular specificity. Nonlinear absorption is a nonlinear optical effect in which the absorption coefficient depends on excitation intensity. As a commonly used spectroscopy tool, nonlinear absorption measurement uncovers many photophysical and photochemical processes correlated with electronic states of molecules. Recently we have been focusing on adapting this spectroscopy method to a microscopy imaging technique. The effort leads to a novel modality in nonlinear microscopy-nonlinear absorption microscopy. This article summarizes the principles and instrumentation of this imaging technique and highlights some of the recent progress in applying it to imaging skin pigmentation and microvasculature under ex vivo or in vivo conditions.

Full Text

Duke Authors

Cited Authors

  • Ye, T; Fu, D; Warren, WS

Published Date

  • May 2009

Published In

Volume / Issue

  • 85 / 3

Start / End Page

  • 631 - 645

PubMed ID

  • 19170931

Pubmed Central ID

  • PMC7341902

Electronic International Standard Serial Number (EISSN)

  • 1751-1097

International Standard Serial Number (ISSN)

  • 0031-8655

Digital Object Identifier (DOI)

  • 10.1111/j.1751-1097.2008.00514.x


  • eng