Skip to main content
Journal cover image

Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles.

Publication ,  Journal Article
Crow, MJ; Seekell, K; Ostrander, JH; Wax, A
Published in: ACS nano
November 2011

The dimerization of receptors on the cell membrane is an important step in the activation of cell signaling pathways. Several methods exist for observing receptor dimerization, including coimmunoprecipitation, chemical cross-linking, and fluorescence resonance energy transfer (FRET). These techniques are limited in that only FRET is appropriate for live cells, but even that method suffers from photobleaching and bleed-through effects. In this study, we implement an alternative method for the targeting of HER-2 homodimer formation based on the plasmonic coupling of gold nanoparticles functionalized with HER-2 Ab. In the presented studies, SK-BR-3 cells, known to overexpress HER-2, are labeled with these nanoparticles and receptor colocalization is observed using plasmonic coupling. HER-2 targeted nanoparticles bound to these cells exhibit a peak resonance that is significantly red-shifted relative to those bound to similar receptors on A549 cells, which have significantly lower levels of HER-2 expression. This significant red shift indicates plasmonic coupling is occurring and points to a new avenue for assessing dimerization by monitoring their colocalization. To determine that dimerization is occurring, the refractive index of the nanoenvironment of the labels is assessed using a theoretical analysis based on the Mie coated sphere model. The results indicate scattering by single, isolated nanoparticles for the low HER-2 expressing A549 cell line, but the scattering observed for the HER-2 overexpressing SK-BR-3 cell line may only be explained by plasmonic-coupling of proximal nanoparticle pairs. To validate the conformation of nanoparticles bound to HER-2 receptors undergoing dimerization, discrete dipole approximation (DDA) models are used to assess spectra of scattering by coupled nanoparticles. Comparison of the experimental results with theoretical models indicates that NP dimers are formed for the labeling of SK-BR-3 cells, suggesting that receptor dimerization has been observed.

Duke Scholars

Published In

ACS nano

DOI

EISSN

1936-086X

ISSN

1936-0851

Publication Date

November 2011

Volume

5

Issue

11

Start / End Page

8532 / 8540

Related Subject Headings

  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Protein Structure, Quaternary
  • Protein Multimerization
  • Nanoscience & Nanotechnology
  • Metal Nanoparticles
  • Humans
  • Gold
  • Gene Expression Regulation
  • Cell Line, Tumor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Crow, M. J., Seekell, K., Ostrander, J. H., & Wax, A. (2011). Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles. ACS Nano, 5(11), 8532–8540. https://doi.org/10.1021/nn201451c
Crow, Matthew J., Kevin Seekell, Julie H. Ostrander, and Adam Wax. “Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles.ACS Nano 5, no. 11 (November 2011): 8532–40. https://doi.org/10.1021/nn201451c.
Crow MJ, Seekell K, Ostrander JH, Wax A. Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles. ACS nano. 2011 Nov;5(11):8532–40.
Crow, Matthew J., et al. “Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles.ACS Nano, vol. 5, no. 11, Nov. 2011, pp. 8532–40. Epmc, doi:10.1021/nn201451c.
Crow MJ, Seekell K, Ostrander JH, Wax A. Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles. ACS nano. 2011 Nov;5(11):8532–8540.
Journal cover image

Published In

ACS nano

DOI

EISSN

1936-086X

ISSN

1936-0851

Publication Date

November 2011

Volume

5

Issue

11

Start / End Page

8532 / 8540

Related Subject Headings

  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Protein Structure, Quaternary
  • Protein Multimerization
  • Nanoscience & Nanotechnology
  • Metal Nanoparticles
  • Humans
  • Gold
  • Gene Expression Regulation
  • Cell Line, Tumor