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¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72.

Publication ,  Journal Article
Werner-Allen, JW; Zhou, P
Published in: Biomol NMR Assign
April 2012

Ssu72 helps regulate transcription and co-transcriptional mRNA processing by dephosphorylating serine residues at the 5th position in the heptad repeats of the C-terminal domain of RNA polymerase II. Here we use multidimensional, multinuclear NMR experiments to assign the backbone and side-chain resonances of the 23 kDa Ssu72 from Drosophila melanogaster in the phosphate-bound state, and use NMR titrations to examine the phosphate-binding properties of three active site mutants.

Duke Scholars

Published In

Biomol NMR Assign

DOI

EISSN

1874-270X

Publication Date

April 2012

Volume

6

Issue

1

Start / End Page

57 / 61

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Protein Tyrosine Phosphatases
  • Nuclear Magnetic Resonance, Biomolecular
  • Drosophila melanogaster
  • Drosophila Proteins
  • Biophysics
  • Binding Sites
  • Animals
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Werner-Allen, J. W., & Zhou, P. (2012). ¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72. Biomol NMR Assign, 6(1), 57–61. https://doi.org/10.1007/s12104-011-9325-2
Werner-Allen, Jon W., and Pei Zhou. “¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72.Biomol NMR Assign 6, no. 1 (April 2012): 57–61. https://doi.org/10.1007/s12104-011-9325-2.
Werner-Allen, Jon W., and Pei Zhou. “¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72.Biomol NMR Assign, vol. 6, no. 1, Apr. 2012, pp. 57–61. Pubmed, doi:10.1007/s12104-011-9325-2.
Journal cover image

Published In

Biomol NMR Assign

DOI

EISSN

1874-270X

Publication Date

April 2012

Volume

6

Issue

1

Start / End Page

57 / 61

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Protein Tyrosine Phosphatases
  • Nuclear Magnetic Resonance, Biomolecular
  • Drosophila melanogaster
  • Drosophila Proteins
  • Biophysics
  • Binding Sites
  • Animals
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology