¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72.

Journal Article (Journal Article)

Ssu72 helps regulate transcription and co-transcriptional mRNA processing by dephosphorylating serine residues at the 5th position in the heptad repeats of the C-terminal domain of RNA polymerase II. Here we use multidimensional, multinuclear NMR experiments to assign the backbone and side-chain resonances of the 23 kDa Ssu72 from Drosophila melanogaster in the phosphate-bound state, and use NMR titrations to examine the phosphate-binding properties of three active site mutants.

Full Text

Duke Authors

Cited Authors

  • Werner-Allen, JW; Zhou, P

Published Date

  • April 2012

Published In

Volume / Issue

  • 6 / 1

Start / End Page

  • 57 - 61

PubMed ID

  • 21732054

Pubmed Central ID

  • PMC3653315

Electronic International Standard Serial Number (EISSN)

  • 1874-270X

Digital Object Identifier (DOI)

  • 10.1007/s12104-011-9325-2


  • eng

Conference Location

  • Netherlands