Changes in bioactive lipids, alkylacylglycerol and ceramide, occur in HIV-infected cells.
Journal Article (Journal Article)
The mass levels of bioactive lipids known to modulate signal transduction or to possess other biological activities were measured in HIV-infected CEM cells. The levels of diacylglycerol, an activator of protein kinase C, as well as of alkylacylglycerol were elevated. A more drastic increase was observed in the ceramide levels after HIV-infection, whereas sphingosine levels were hardly influenced. Interestingly, the magnitude of the changes was related to the infection time, being higher at 8 days after infection then at 4 days. The possible role of these lipids in the cytopathic effects of HIV-infection is discussed. In addition, an improved methodology to quantitate simultaneously diacylglycerol and alkylacylglycerol in crude lipid extracts, based upon their phosphorylation by E. coli diacylglycerol kinase, is presented.
- Van Veldhoven, PP; Matthews, TJ; Bolognesi, DP; Bell, RM
- August 31, 1992
Volume / Issue
- 187 / 1
Start / End Page
- 209 - 216
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States