Broadly neutralizing antibodies elicited by the hypervariable neutralizing determinant of HIV-1.

Published

Journal Article

The principal neutralizing determinant (PND) of human immunodeficiency virus (HIV)-1 resides within the V3 loop of the envelope protein. Antibodies elicited by peptides of this region were able to neutralize diverse isolates. Serum from one of three animals immunized with the human T cell lymphoma virus (HTLV)-IIIMN PND peptide, RP142, neutralized MN and the sequence-divergent HTLV-IIIB isolate. Serum from one of three animals immunized with a 13-amino acid IIIB PND peptide (RP337) also neutralized both of these isolates. Characterization of these sera revealed that the cross-neutralizing antibodies bound the amino acid sequence GlyProGlyArgAlaPhe (GPGRAF) that is present in both isolates. This sequence is frequently found in the PNDs analyzed in randomly selected HIV-1 isolates. Sera from two rabbits immunized with a peptide containing only the GPGRAF residues neutralized divergent isolates, including IIIB and MN.

Full Text

Duke Authors

Cited Authors

  • Javaherian, K; Langlois, AJ; LaRosa, GJ; Profy, AT; Bolognesi, DP; Herlihy, WC; Putney, SD; Matthews, TJ

Published Date

  • December 1990

Published In

Volume / Issue

  • 250 / 4987

Start / End Page

  • 1590 - 1593

PubMed ID

  • 1703322

Pubmed Central ID

  • 1703322

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1703322

Language

  • eng