Principal neutralizing domain of the human immunodeficiency virus type 1 envelope protein.

Published

Journal Article

The principal neutralizing determinant of human immunodeficiency virus type 1 (HIV-1) is located in the external envelope protein, gp120, and has previously been mapped to a 24-amino acid-long sequence (denoted RP135). We show here that deletion of this sequence renders the envelope unable to elicit neutralizing antibodies. In addition, using synthetic peptide fragments of RP135, we have mapped the neutralizing determinant to 8 amino acids and found that a peptide of this size elicits neutralizing antibodies. This sequence contains a central Gly-Pro-Gly that is generally conserved between different HIV-1 isolates and is flanked by amino acids that differ from isolate to isolate. Antibodies elicited by peptides from one isolate do not neutralize two different isolates, and a hybrid peptide, consisting of amino acid sequences from two isolates, elicits neutralizing antibodies to both isolates. By using a mixture of peptides of this domain or a mixture of such hybrid peptides the type-specificity of the neutralizing antibody response to this determinant can perhaps be overcome.

Full Text

Duke Authors

Cited Authors

  • Javaherian, K; Langlois, AJ; McDanal, C; Ross, KL; Eckler, LI; Jellis, CL; Profy, AT; Rusche, JR; Bolognesi, DP; Putney, SD

Published Date

  • September 1989

Published In

Volume / Issue

  • 86 / 17

Start / End Page

  • 6768 - 6772

PubMed ID

  • 2771954

Pubmed Central ID

  • 2771954

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.86.17.6768

Language

  • eng

Conference Location

  • United States