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Functional reentry in cultured monolayers of neonatal rat cardiac cells.

Publication ,  Journal Article
Iravanian, S; Nabutovsky, Y; Kong, C-R; Saha, S; Bursac, N; Tung, L
Published in: American journal of physiology. Heart and circulatory physiology
July 2003

Previous studies of reentrant arrhythmias in the heart have been performed in computer models and tissue experiments. We hypothesized that confluent monolayers of cardiac cells can provide a simple, controlled, and reproducible experimental model of reentry. Neonatal rat ventricular cells were cultured on 22-mm-diameter coverslips and stained with the voltage-sensitive dye RH-237. Recordings of transmembrane potentials were obtained from 61 sites with the use of a contact fluorescence imaging system. An electrical field stimulus, followed by a point stimulus, induced 39 episodes of sustained reentry and 21 episodes of nonsustained reentry. Sustained reentry consisted of single-loop (n = 18 monolayers) or figure-of-eight (n = 4) patterns. The cycle length, action potential duration at 80% repolarization, and conduction velocity were (in means +/- SE) 358 +/- 33 ms, 118 +/- 12 ms, and 12.9 +/- 1.0 cm/s for single loop and 311 +/- 78 ms, 137 +/- 18 ms, and 7.8 +/- 1.3 cm/s for figure-of-eight, respectively. Electrical termination by 6- to 13-V/cm field pulses or 15- to 20-V point stimuli was successful in 60% of the attempts. In summary, highly stable reentry can be induced, sustained for extensive periods of time, and electrically terminated in monolayers of cultured neonatal rat cardiac myocytes.

Duke Scholars

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

July 2003

Volume

285

Issue

1

Start / End Page

H449 / H456

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Patch-Clamp Techniques
  • Myocardium
  • Heart
  • Fluorescent Dyes
  • Electrophysiology
  • Electrocardiography
  • Electric Stimulation
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Iravanian, S., Nabutovsky, Y., Kong, C.-R., Saha, S., Bursac, N., & Tung, L. (2003). Functional reentry in cultured monolayers of neonatal rat cardiac cells. American Journal of Physiology. Heart and Circulatory Physiology, 285(1), H449–H456. https://doi.org/10.1152/ajpheart.00896.2002
Iravanian, Shahriar, Yelena Nabutovsky, Chae-Ryon Kong, Sumita Saha, Nenad Bursac, and Leslie Tung. “Functional reentry in cultured monolayers of neonatal rat cardiac cells.American Journal of Physiology. Heart and Circulatory Physiology 285, no. 1 (July 2003): H449–56. https://doi.org/10.1152/ajpheart.00896.2002.
Iravanian S, Nabutovsky Y, Kong C-R, Saha S, Bursac N, Tung L. Functional reentry in cultured monolayers of neonatal rat cardiac cells. American journal of physiology Heart and circulatory physiology. 2003 Jul;285(1):H449–56.
Iravanian, Shahriar, et al. “Functional reentry in cultured monolayers of neonatal rat cardiac cells.American Journal of Physiology. Heart and Circulatory Physiology, vol. 285, no. 1, July 2003, pp. H449–56. Epmc, doi:10.1152/ajpheart.00896.2002.
Iravanian S, Nabutovsky Y, Kong C-R, Saha S, Bursac N, Tung L. Functional reentry in cultured monolayers of neonatal rat cardiac cells. American journal of physiology Heart and circulatory physiology. 2003 Jul;285(1):H449–H456.

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

July 2003

Volume

285

Issue

1

Start / End Page

H449 / H456

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Patch-Clamp Techniques
  • Myocardium
  • Heart
  • Fluorescent Dyes
  • Electrophysiology
  • Electrocardiography
  • Electric Stimulation
  • Cells, Cultured