Cardiac muscle tissue engineering: toward an in vitro model for electrophysiological studies.

Published

Journal Article

The objective of this study was to establish a three-dimensional (3-D) in vitro model system of cardiac muscle for electrophysiological studies. Primary neonatal rat ventricular cells containing lower or higher fractions of cardiac myocytes were cultured on polymeric scaffolds in bioreactors to form regular or enriched cardiac muscle constructs, respectively. After 1 wk, all constructs contained a peripheral tissue-like region (50-70 micrometer thick) in which differentiated cardiac myocytes were organized in multiple layers in a 3-D configuration. Indexes of cell size (protein/DNA) and metabolic activity (tetrazolium conversion/DNA) were similar for constructs and neonatal rat ventricles. Electrophysiological studies conducted using a linear array of extracellular electrodes showed that the peripheral region of constructs exhibited relatively homogeneous electrical properties and sustained macroscopically continuous impulse propagation on a centimeter-size scale. Electrophysiological properties of enriched constructs were superior to those of regular constructs but inferior to those of native ventricles. These results demonstrate that 3-D cardiac muscle constructs can be engineered with cardiac-specific structural and electrophysiological properties and used for in vitro impulse propagation studies.

Full Text

Duke Authors

Cited Authors

  • Bursac, N; Papadaki, M; Cohen, RJ; Schoen, FJ; Eisenberg, SR; Carrier, R; Vunjak-Novakovic, G; Freed, LE

Published Date

  • August 1999

Published In

Volume / Issue

  • 277 / 2

Start / End Page

  • H433 - H444

PubMed ID

  • 10444466

Pubmed Central ID

  • 10444466

Electronic International Standard Serial Number (EISSN)

  • 2163-5773

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajpheart.1999.277.2.h433

Language

  • eng