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Expression and amplification of the HER-2/neu (c-erbB-2) protooncogene in epithelial ovarian tumors and cell lines.

Publication ,  Journal Article
Tyson, FL; Boyer, CM; Kaufman, R; O'Briant, K; Cram, G; Crews, JR; Soper, JT; Daly, L; Fowler, WC; Haskill, JS
Published in: Am J Obstet Gynecol
September 1991

Amplification of the c-erbB-2 protooncogene has been associated with a poor prognosis in human breast and ovarian cancers. Our study was undertaken to examine whether amplification, rearrangement, or overexpression of c-erbB-2 and other protooncogenes was frequently observed in epithelial ovarian cancers. c-erbB-2 was expressed in 87% of 22 ovarian cancers analyzed, but expression was significantly increased in only one of the 22 tumor specimens. In this case elevated c-erbB-2 expression was associated with dramatic amplification of the gene. In another tumor a 3.8 kb EcoRI fragment was found, in addition to the usual 4.4 and 6.0 kb fragments; this is consistent with a possible gene rearrangement or a restriction fragment length polymorphism. To place these results in perspective, expression of several other protooncogenes has been examined in ovarian carcinomas. The c-fos, c-myc, n-myc, c-fms, and c-Ha-ras protooncogenes were expressed in different fractions of tumors, but expression of l-myc, c-erbB, c-myb, c-sis, and c-mos was not detectable. Aside from c-erbB-2, neither amplification nor rearrangement was observed among the other protooncogenes studied. Expression of c-erbB-2, c-fms, c-myc, n-myc, c-fos, and c-Ha-ras deserves further evaluation as a prognostic factor in ovarian cancer.

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Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

September 1991

Volume

165

Issue

3

Start / End Page

640 / 646

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • RNA, Neoplasm
  • Proto-Oncogenes
  • Proto-Oncogene Proteins
  • Polymorphism, Restriction Fragment Length
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Macrophage Colony-Stimulating Factor
 

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Tyson, F. L., Boyer, C. M., Kaufman, R., O’Briant, K., Cram, G., Crews, J. R., … Haskill, J. S. (1991). Expression and amplification of the HER-2/neu (c-erbB-2) protooncogene in epithelial ovarian tumors and cell lines. Am J Obstet Gynecol, 165(3), 640–646. https://doi.org/10.1016/0002-9378(91)90300-g
Tyson, F. L., C. M. Boyer, R. Kaufman, K. O’Briant, G. Cram, J. R. Crews, J. T. Soper, L. Daly, W. C. Fowler, and J. S. Haskill. “Expression and amplification of the HER-2/neu (c-erbB-2) protooncogene in epithelial ovarian tumors and cell lines.Am J Obstet Gynecol 165, no. 3 (September 1991): 640–46. https://doi.org/10.1016/0002-9378(91)90300-g.
Tyson FL, Boyer CM, Kaufman R, O’Briant K, Cram G, Crews JR, et al. Expression and amplification of the HER-2/neu (c-erbB-2) protooncogene in epithelial ovarian tumors and cell lines. Am J Obstet Gynecol. 1991 Sep;165(3):640–6.
Tyson, F. L., et al. “Expression and amplification of the HER-2/neu (c-erbB-2) protooncogene in epithelial ovarian tumors and cell lines.Am J Obstet Gynecol, vol. 165, no. 3, Sept. 1991, pp. 640–46. Pubmed, doi:10.1016/0002-9378(91)90300-g.
Tyson FL, Boyer CM, Kaufman R, O’Briant K, Cram G, Crews JR, Soper JT, Daly L, Fowler WC, Haskill JS. Expression and amplification of the HER-2/neu (c-erbB-2) protooncogene in epithelial ovarian tumors and cell lines. Am J Obstet Gynecol. 1991 Sep;165(3):640–646.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

September 1991

Volume

165

Issue

3

Start / End Page

640 / 646

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • RNA, Neoplasm
  • Proto-Oncogenes
  • Proto-Oncogene Proteins
  • Polymorphism, Restriction Fragment Length
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Macrophage Colony-Stimulating Factor