Biochemical and immunological properties of dog brain isorenin.

Journal Article (Journal Article)

A neutral protease with angiotensin I-forming activity which could readily be separated from acid proteases and plasma and renal renin was obtained from extracts of dog brain. This enzyme has an apparent mol wt of 40,000 by Sephadex chromatography. On chromatofocusing, it displays isoelectric points of 7.92, 7.73, and 7.42, and thus, it is a basic protein, in contrast to either renal or plasma renin which are acidic proteins. This brain enzyme does not react with antibodies specific for dog kidney renin. Since the brain enzyme forms angiotensin I from renin substrate at neutral pH, yet can be separated from and has isoelectric points different from renal renin, it is an isoenzyme of the kidney counterpart. The majority of the renin-like activity of dog brain is due to this isoenzyme.

Full Text

Duke Authors

Cited Authors

  • Husain, A; Smeby, RR; Wilk, D; Dzau, VJ; Bumpus, FM

Published Date

  • June 1984

Published In

Volume / Issue

  • 114 / 6

Start / End Page

  • 2210 - 2215

PubMed ID

  • 6373240

International Standard Serial Number (ISSN)

  • 0013-7227

Digital Object Identifier (DOI)

  • 10.1210/endo-114-6-2210


  • eng

Conference Location

  • United States