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Biochemical and immunological properties of dog brain isorenin.

Publication ,  Journal Article
Husain, A; Smeby, RR; Wilk, D; Dzau, VJ; Bumpus, FM
Published in: Endocrinology
June 1984

A neutral protease with angiotensin I-forming activity which could readily be separated from acid proteases and plasma and renal renin was obtained from extracts of dog brain. This enzyme has an apparent mol wt of 40,000 by Sephadex chromatography. On chromatofocusing, it displays isoelectric points of 7.92, 7.73, and 7.42, and thus, it is a basic protein, in contrast to either renal or plasma renin which are acidic proteins. This brain enzyme does not react with antibodies specific for dog kidney renin. Since the brain enzyme forms angiotensin I from renin substrate at neutral pH, yet can be separated from and has isoelectric points different from renal renin, it is an isoenzyme of the kidney counterpart. The majority of the renin-like activity of dog brain is due to this isoenzyme.

Duke Scholars

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

June 1984

Volume

114

Issue

6

Start / End Page

2210 / 2215

Location

United States

Related Subject Headings

  • Renin
  • Organ Specificity
  • Nephrectomy
  • Male
  • Kidney
  • Immune Sera
  • Female
  • Endopeptidases
  • Endocrinology & Metabolism
  • Dogs
 

Citation

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Husain, A., Smeby, R. R., Wilk, D., Dzau, V. J., & Bumpus, F. M. (1984). Biochemical and immunological properties of dog brain isorenin. Endocrinology, 114(6), 2210–2215. https://doi.org/10.1210/endo-114-6-2210
Husain, A., R. R. Smeby, D. Wilk, V. J. Dzau, and F. M. Bumpus. “Biochemical and immunological properties of dog brain isorenin.Endocrinology 114, no. 6 (June 1984): 2210–15. https://doi.org/10.1210/endo-114-6-2210.
Husain A, Smeby RR, Wilk D, Dzau VJ, Bumpus FM. Biochemical and immunological properties of dog brain isorenin. Endocrinology. 1984 Jun;114(6):2210–5.
Husain, A., et al. “Biochemical and immunological properties of dog brain isorenin.Endocrinology, vol. 114, no. 6, June 1984, pp. 2210–15. Pubmed, doi:10.1210/endo-114-6-2210.
Husain A, Smeby RR, Wilk D, Dzau VJ, Bumpus FM. Biochemical and immunological properties of dog brain isorenin. Endocrinology. 1984 Jun;114(6):2210–2215.
Journal cover image

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

June 1984

Volume

114

Issue

6

Start / End Page

2210 / 2215

Location

United States

Related Subject Headings

  • Renin
  • Organ Specificity
  • Nephrectomy
  • Male
  • Kidney
  • Immune Sera
  • Female
  • Endopeptidases
  • Endocrinology & Metabolism
  • Dogs