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Use of viral systems to study miRNA-mediated regulation of gene expression in human cells.

Publication ,  Journal Article
Forte, E; Luftig, MA
Published in: Methods Mol Biol
2013

MicroRNAs (miRNAs) are a class of small ∼22 nt regulatory RNAs that modulate mRNA expression in all multicellular eukaryotic organisms. Interestingly, viruses also encode miRNAs and these viral miRNAs target cellular and viral mRNAs to regulate virus replication and latent infection. In particular, herpesviruses encode a large number of miRNAs. Herpesvirus infection also changes the normal expression profile of cellular miRNAs. New genetic tools have recently been generated to study the function of viral and cellular miRNAs in virus-infected cells. The creation of these reagents and use in Epstein-Barr virus-infected lymphoblastoid cell lines are discussed as a model viral system for the investigation of miRNA function.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

936

Start / End Page

143 / 156

Location

United States

Related Subject Headings

  • Transduction, Genetic
  • Retroviridae
  • Real-Time Polymerase Chain Reaction
  • MicroRNAs
  • Humans
  • Genetic Vectors
  • Genes, Reporter
  • Gene Order
  • Gene Expression Regulation
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Forte, E., & Luftig, M. A. (2013). Use of viral systems to study miRNA-mediated regulation of gene expression in human cells. Methods Mol Biol, 936, 143–156. https://doi.org/10.1007/978-1-62703-083-0_12
Forte, Eleonora, and Micah A. Luftig. “Use of viral systems to study miRNA-mediated regulation of gene expression in human cells.Methods Mol Biol 936 (2013): 143–56. https://doi.org/10.1007/978-1-62703-083-0_12.
Forte, Eleonora, and Micah A. Luftig. “Use of viral systems to study miRNA-mediated regulation of gene expression in human cells.Methods Mol Biol, vol. 936, 2013, pp. 143–56. Pubmed, doi:10.1007/978-1-62703-083-0_12.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

936

Start / End Page

143 / 156

Location

United States

Related Subject Headings

  • Transduction, Genetic
  • Retroviridae
  • Real-Time Polymerase Chain Reaction
  • MicroRNAs
  • Humans
  • Genetic Vectors
  • Genes, Reporter
  • Gene Order
  • Gene Expression Regulation
  • Developmental Biology