Atomic force microscopy captures folded ribosome bound nascent chains.

Direct visualization of co-translational folding of nascent polypeptide chains is challenging. Here we present, for the first time, AFM images of large protein constructs based on the membrane binding domain of ankyrin-R, complexed with the ribosome. The characteristic "horse-shoe" shape of ankyrin-R emerging from the ribosome was captured.

Full Text

Duke Authors

Cited Authors

  • Loksztejn, A; Scholl, Z; Marszalek, PE

Published Date

  • December 14, 2012

Published In

Volume / Issue

  • 48 / 96

Start / End Page

  • 11727 - 11729

PubMed ID

  • 23051696

Electronic International Standard Serial Number (EISSN)

  • 1364-548X

Digital Object Identifier (DOI)

  • 10.1039/c2cc35551e

Language

  • eng

Citation Source

  • PubMed