Increased expression of sialyl-dimeric LeX antigen in liver metastases of human colorectal carcinoma.

Published

Journal Article

We collected a total of 78 tissue specimens, including primary colorectal carcinoma, normal colonic mucosa, and liver metastases of colon carcinoma, to examine whether the extracts of these tissues inhibited the binding of a monoclonal antibody FH6, specific for sialyl-dimeric LeX antigen. The results of inhibition assays demonstrated that: (a) contents of FH6-reactive molecules were greater in carcinoma tissues than in normal colonic mucosa; (b) metastatic foci in livers contained more FH6-reactive molecules than primary tumors; (c) primary tumors from Dukes' stage B1 patients contained less FH6-reactive molecules than primary tumors from Dukes' stage D patients. The inhibitory activity of these tumor tissue extracts against the binding of a monoclonal antibody FH6 to cultured colon carcinoma cells was eliminated by prior treatment of the extracts with sialidase, confirming that the FH6-reactive materials were sialyl-dimeric LeX antigen. Electrophoretic separation of tumor tissue extracts on 3% polyacrylamide gels followed by direct staining with monoclonal antibody FH6 revealed that very high molecular weight glycoproteins, presumably mucins, contained sialyl-dimeric LeX antigen.

Full Text

Duke Authors

Cited Authors

  • Hoff, SD; Matsushita, Y; Ota, DM; Cleary, KR; Yamori, T; Hakomori, S; Irimura, T

Published Date

  • December 15, 1989

Published In

Volume / Issue

  • 49 / 24 Pt 1

Start / End Page

  • 6883 - 6888

PubMed ID

  • 2573422

Pubmed Central ID

  • 2573422

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States