Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.

Journal Article (Journal Article)

We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.

Full Text

Duke Authors

Cited Authors

  • Reller, ME; Mallonee, AB; Kwiatkowski, NP; Merz, WG

Published Date

  • November 2007

Published In

Volume / Issue

  • 45 / 11

Start / End Page

  • 3802 - 3803

PubMed ID

  • 17804657

Pubmed Central ID

  • PMC2168530

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/JCM.01127-07

Language

  • eng

Conference Location

  • United States