Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.
Journal Article (Journal Article)
We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.
Full Text
Duke Authors
Cited Authors
- Reller, ME; Mallonee, AB; Kwiatkowski, NP; Merz, WG
Published Date
- November 2007
Published In
Volume / Issue
- 45 / 11
Start / End Page
- 3802 - 3803
PubMed ID
- 17804657
Pubmed Central ID
- PMC2168530
International Standard Serial Number (ISSN)
- 0095-1137
Digital Object Identifier (DOI)
- 10.1128/JCM.01127-07
Language
- eng
Conference Location
- United States