Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.
Publication
, Journal Article
Reller, ME; Mallonee, AB; Kwiatkowski, NP; Merz, WG
Published in: J Clin Microbiol
November 2007
We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.
Duke Scholars
Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
November 2007
Volume
45
Issue
11
Start / End Page
3802 / 3803
Location
United States
Related Subject Headings
- Peptide Nucleic Acids
- Nucleic Acid Probes
- Microbiology
- In Situ Hybridization, Fluorescence
- Humans
- Candida
- 11 Medical and Health Sciences
- 07 Agricultural and Veterinary Sciences
- 06 Biological Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Reller, M. E., Mallonee, A. B., Kwiatkowski, N. P., & Merz, W. G. (2007). Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol, 45(11), 3802–3803. https://doi.org/10.1128/JCM.01127-07
Reller, Megan E., Amanda B. Mallonee, Nicole P. Kwiatkowski, and William G. Merz. “Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.” J Clin Microbiol 45, no. 11 (November 2007): 3802–3. https://doi.org/10.1128/JCM.01127-07.
Reller ME, Mallonee AB, Kwiatkowski NP, Merz WG. Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol. 2007 Nov;45(11):3802–3.
Reller, Megan E., et al. “Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.” J Clin Microbiol, vol. 45, no. 11, Nov. 2007, pp. 3802–03. Pubmed, doi:10.1128/JCM.01127-07.
Reller ME, Mallonee AB, Kwiatkowski NP, Merz WG. Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol. 2007 Nov;45(11):3802–3803.
Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
November 2007
Volume
45
Issue
11
Start / End Page
3802 / 3803
Location
United States
Related Subject Headings
- Peptide Nucleic Acids
- Nucleic Acid Probes
- Microbiology
- In Situ Hybridization, Fluorescence
- Humans
- Candida
- 11 Medical and Health Sciences
- 07 Agricultural and Veterinary Sciences
- 06 Biological Sciences