Characterization of myosin heavy chain by cyanogen bromide peptide maps.

Journal Article (Journal Article)

Procedures have been developed for the preparation of pure myosin heavy chain (h-myosin) by preparative gel electrophoresis, and for the characterization of h-myosin by cyanogen bromide peptide mapping. Major sources of error are the oxidation of methionine and the proteolytic splitting of the chain during purification. These errors have been eliminated. A peculiar feature is the doubling or quadrupling of a peptide of molecular weight 17 000. The results show structural differences between isomyosins derived from myonal types within the same animal, as well as interspecies differences.

Full Text

Duke Authors

Cited Authors

  • Mommaerts, WF; Vegh, K; Seraydarian, K; Meier, K; Rittschof, D

Published Date

  • June 1, 1982

Published In

Volume / Issue

  • 3 / 2

Start / End Page

  • 129 - 144

PubMed ID

  • 7107865

Electronic International Standard Serial Number (EISSN)

  • 1573-2657

International Standard Serial Number (ISSN)

  • 0142-4319

Digital Object Identifier (DOI)

  • 10.1007/bf00711938


  • eng