High potency silencing by single-stranded boranophosphate siRNA.
Journal Article (Journal Article)
In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and research applications, its overall poor activity compared with ds-siRNA has prevented its widespread use. In contrast to the poor gene silencing activity of native ss-siRNA, we found that the silencing activity of boranophosphate-modified ss-siRNA is comparable with that of unmodified ds-siRNA. Boranophosphate ss-siRNA has excellent maximum silencing activity and is highly effective at low concentrations. The silencing activity of boranophosphate ss-siRNA is also durable, with significant silencing up to 1 week after transfection. Thus, we have demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-siRNA, suggesting that boranophosphate-modified ss-siRNAs should be investigated as a potential new class of therapeutic agents.
Full Text
Duke Authors
Cited Authors
- Hall, AHS; Wan, J; Spesock, A; Sergueeva, Z; Shaw, BR; Alexander, KA
Published Date
- 2006
Published In
Volume / Issue
- 34 / 9
Start / End Page
- 2773 - 2781
PubMed ID
- 16717282
Pubmed Central ID
- PMC1464415
Electronic International Standard Serial Number (EISSN)
- 1362-4962
Digital Object Identifier (DOI)
- 10.1093/nar/gkl339
Language
- eng
Conference Location
- England