Detection of phosphorylation by enzymatic techniques.

Published

Journal Article

This unit presents protocols for examining the functional effects elicited by nonspecific acid or alkaline phosphatases that dephosphorylate many phosphoproteins in vitro. Additional protocols describe digestion of phosphoproteins with a protein serine/threonine phosphatase and protein tyrosine phosphatase. A support protocol has been included to identify the radiolabel as (32)P(I) based on its ability to form a complex with ammonium molybdate.

Full Text

Duke Authors

Cited Authors

  • Shenolikar, S

Published Date

  • May 2001

Published In

Volume / Issue

  • Chapter 13 /

Start / End Page

  • Unit13.5 -

PubMed ID

  • 18429117

Pubmed Central ID

  • 18429117

Electronic International Standard Serial Number (EISSN)

  • 1934-3663

International Standard Serial Number (ISSN)

  • 1934-3655

Digital Object Identifier (DOI)

  • 10.1002/0471140864.ps1305s05

Language

  • eng