The mechanisms underlying the spatial spreading of signaling activity.

Journal Article (Journal Article;Review)

During the induction of plasticity of dendritic spines, many intracellular signaling pathways are spatially and temporally regulated to co-ordinate downstream cellular processes in different dendritic micron-domains. Recent advent of imaging technology based on fluorescence resonance energy transfer (FRET) has allowed the direct monitoring of the spatiotemporal regulation of signaling activity in spines and dendrites during synaptic plasticity. In particular, the activity of three small GTPase proteins HRas, Cdc42, and RhoA, which share similar structure and mobility on the plasma membrane, displayed different spatial spreading patterns: Cdc42 is compartmentalized in the stimulated spines while RhoA and HRas spread into dendrites over 5-10 μm. These measurements thus provide the basis for understanding the mechanisms underlying the spatiotemporal regulation of signaling activity. Further, using spatiotemporally controlled spine stimulations, some of the roles of signal spreading have been revealed.

Full Text

Duke Authors

Cited Authors

  • Yasuda, R; Murakoshi, H

Published Date

  • April 2011

Published In

Volume / Issue

  • 21 / 2

Start / End Page

  • 313 - 321

PubMed ID

  • 21429735

Pubmed Central ID

  • PMC3111059

Electronic International Standard Serial Number (EISSN)

  • 1873-6882

Digital Object Identifier (DOI)

  • 10.1016/j.conb.2011.02.008


  • eng

Conference Location

  • England