Dibutyryl cyclic adenosine monophosphate reduces expression of c-myc during HL-60 differentiation.

Journal Article (Journal Article)

The human promyelocytic leukemia cell line HL-60 is induced to differentiate along a myelocytic pathway by dibutyryl cyclic adenosine monophosphate (dbcAMP). Other cAMP analogs are ineffective as inducing agents. The effect of these compounds on expression of c-myc was investigated using a DNA probe for c-myc to detect RNA transcripts. The dose response and time to commitment for reduction in c-myc expression with dbcAMP was similar to the findings for phenotypic changes. Bromo-cyclic AMP and butyrate alone caused no changes in c-myc expression in 24 hours, but demonstrated dramatic synergism together, suggesting that butyrate contributes in part to the effects of dbcAMP. Evidence for mechanisms of action of cAMP other than activation of the cAMP-dependent protein kinase is reviewed.

Full Text

Duke Authors

Cited Authors

  • McCachren, SS; Nichols, J; Kaufman, RE; Niedel, JE

Published Date

  • August 1986

Published In

Volume / Issue

  • 68 / 2

Start / End Page

  • 412 - 416

PubMed ID

  • 3015282

International Standard Serial Number (ISSN)

  • 0006-4971


  • eng

Conference Location

  • United States