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gamma-Aminobutyric acid and benzodiazepine receptors in the kindling model of epilepsy: a quantitative radiohistochemical study.

Publication ,  Journal Article
Shin, C; Pedersen, HB; McNamara, JO
Published in: J Neurosci
October 1985

Quantitative radiohistochemistry was utilized to study alterations of gamma-aminobutyric acid (GABA) and benzodiazepine receptors in the kindling model of epilepsy. The radioligands used for GABA and benzodiazepine receptors were [3H] muscimol and [3H]flunitrazepam, respectively. GABA receptor binding was increased by 22% in fascia dentata of the hippocampal formation but not in neocortex or substantia nigra of kindled rats. Within fascia dentata, GABA receptor binding was increased to an equivalent extent in stratum granulosum and throughout stratum moleculare; no increase was found in dentate hilus or stratum lacunosummoleculare or stratum radiatum of CA1. The increased binding was present at 24 hr but not at 28 days after the last kindled seizure. The direction, anatomic distribution, and time course of the increased GABA receptor binding were paralleled by increased benzodiazepine receptor binding. Unexpectedly, GABA receptor-mediated enhancement of benzodiazepine receptor binding was slightly attenuated in fascia dentata of kindled compared to control rats. The anatomic distribution of the increased GABA receptor binding is consistent with a localization to somata and dendritic trees of dentate granule cells. We suggest that increased GABA and benzodiazepine receptor binding may contribute to enhanced inhibition of dentate granule cells demonstrated electrophysiologically in kindled animals.

Duke Scholars

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

October 1985

Volume

5

Issue

10

Start / End Page

2696 / 2701

Location

United States

Related Subject Headings

  • Tritium
  • Tissue Distribution
  • Receptors, GABA-A
  • Rats, Inbred Strains
  • Rats
  • Neurology & Neurosurgery
  • Muscimol
  • Male
  • Kindling, Neurologic
  • Histocytochemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shin, C., Pedersen, H. B., & McNamara, J. O. (1985). gamma-Aminobutyric acid and benzodiazepine receptors in the kindling model of epilepsy: a quantitative radiohistochemical study. J Neurosci, 5(10), 2696–2701. https://doi.org/10.1523/JNEUROSCI.05-10-02696.1985
Shin, C., H. B. Pedersen, and J. O. McNamara. “gamma-Aminobutyric acid and benzodiazepine receptors in the kindling model of epilepsy: a quantitative radiohistochemical study.J Neurosci 5, no. 10 (October 1985): 2696–2701. https://doi.org/10.1523/JNEUROSCI.05-10-02696.1985.
Shin, C., et al. “gamma-Aminobutyric acid and benzodiazepine receptors in the kindling model of epilepsy: a quantitative radiohistochemical study.J Neurosci, vol. 5, no. 10, Oct. 1985, pp. 2696–701. Pubmed, doi:10.1523/JNEUROSCI.05-10-02696.1985.

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

October 1985

Volume

5

Issue

10

Start / End Page

2696 / 2701

Location

United States

Related Subject Headings

  • Tritium
  • Tissue Distribution
  • Receptors, GABA-A
  • Rats, Inbred Strains
  • Rats
  • Neurology & Neurosurgery
  • Muscimol
  • Male
  • Kindling, Neurologic
  • Histocytochemistry