Molecular sentinel-on-chip for SERS-based biosensing.
Journal Article (Journal Article)
The development of DNA detection techniques on large-area plasmonics-active platforms is critical for many medical applications such as high-throughput screening, medical diagnosis and systems biology research. Here, we report for the first time a unique "molecular sentinel-on-chip" (MSC) technology for surface-enhanced Raman scattering (SERS)-based DNA detection. This unique approach allows label-free detection of DNA molecules on chips developed on a wafer scale using large area nanofabrication methodologies. To develop plasmonics-active biosensing platforms in a repeatable and reproducible manner, we employed a combination of deep UV lithography, atomic layer deposition, and metal deposition to fabricate triangular-shaped nanowire (TSNW) arrays having controlled sub-10 nm gap nanostructures over an entire 6 inch wafer. The detection of a DNA sequence of the Ki-67 gene, a critical breast cancer biomarker, on the TSNW substrate illustrates the usefulness and potential of the MSC technology as a novel SERS-based DNA detection method.
Full Text
Duke Authors
Cited Authors
- Wang, H-N; Dhawan, A; Du, Y; Batchelor, D; Leonard, DN; Misra, V; Vo-Dinh, T
Published Date
- April 2013
Published In
Volume / Issue
- 15 / 16
Start / End Page
- 6008 - 6015
PubMed ID
- 23493773
Pubmed Central ID
- PMC4022304
Electronic International Standard Serial Number (EISSN)
- 1463-9084
International Standard Serial Number (ISSN)
- 1463-9076
Digital Object Identifier (DOI)
- 10.1039/c3cp00076a
Language
- eng