Molecular sentinel-on-chip for SERS-based biosensing.

Journal Article (Journal Article)

The development of DNA detection techniques on large-area plasmonics-active platforms is critical for many medical applications such as high-throughput screening, medical diagnosis and systems biology research. Here, we report for the first time a unique "molecular sentinel-on-chip" (MSC) technology for surface-enhanced Raman scattering (SERS)-based DNA detection. This unique approach allows label-free detection of DNA molecules on chips developed on a wafer scale using large area nanofabrication methodologies. To develop plasmonics-active biosensing platforms in a repeatable and reproducible manner, we employed a combination of deep UV lithography, atomic layer deposition, and metal deposition to fabricate triangular-shaped nanowire (TSNW) arrays having controlled sub-10 nm gap nanostructures over an entire 6 inch wafer. The detection of a DNA sequence of the Ki-67 gene, a critical breast cancer biomarker, on the TSNW substrate illustrates the usefulness and potential of the MSC technology as a novel SERS-based DNA detection method.

Full Text

Duke Authors

Cited Authors

  • Wang, H-N; Dhawan, A; Du, Y; Batchelor, D; Leonard, DN; Misra, V; Vo-Dinh, T

Published Date

  • April 2013

Published In

Volume / Issue

  • 15 / 16

Start / End Page

  • 6008 - 6015

PubMed ID

  • 23493773

Pubmed Central ID

  • PMC4022304

Electronic International Standard Serial Number (EISSN)

  • 1463-9084

International Standard Serial Number (ISSN)

  • 1463-9076

Digital Object Identifier (DOI)

  • 10.1039/c3cp00076a


  • eng