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Quantifying melanin spatial distribution using pump-probe microscopy and a 2-D morphological autocorrelation transformation for melanoma diagnosis.

Publication ,  Journal Article
Robles, FE; Wilson, JW; Warren, WS
Published in: Journal of biomedical optics
December 2013

Pump-probe microscopy is an emerging molecular imaging technique that probes the excited state dynamics properties of pigmented samples. This method has been particularly intriguing for melanoma because, unlike other methods available, it can provide nondestructive, quantitative chemical information regarding different types of melanins, with high spatial resolution. In this Letter, we present a method based on mathematical morphology to quantify melanin structure (eumelanin, pheomelanin, and total melanin content, uniquely available with pump-probe microscopy) to aid in melanoma diagnosis. The approach applies a two-dimensional autocorrelation function and utilizes statistical parameters of the corresponding autocorrelation images, specifically, the second moments and entropy, to parameterize image structure. Along with bulk melanin chemical information, we show that this method can differentiate invasive melanomas from noninvasive and benign lesions with high sensitivity and specificity (92.3% and 97.5%, respectively, with N=53). The mathematical method and the statistical analysis are described in detail and results from cutaneous and ocular conjunctival melanocytic lesions are presented.

Duke Scholars

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

December 2013

Volume

18

Issue

12

Start / End Page

120502

Related Subject Headings

  • Skin Neoplasms
  • Skin
  • Optics
  • Microscopy
  • Melanoma
  • Melanins
  • Image Processing, Computer-Assisted
  • Humans
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
 

Citation

APA
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ICMJE
MLA
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Robles, F. E., Wilson, J. W., & Warren, W. S. (2013). Quantifying melanin spatial distribution using pump-probe microscopy and a 2-D morphological autocorrelation transformation for melanoma diagnosis. Journal of Biomedical Optics, 18(12), 120502. https://doi.org/10.1117/1.jbo.18.12.120502
Robles, Francisco E., Jesse W. Wilson, and Warren S. Warren. “Quantifying melanin spatial distribution using pump-probe microscopy and a 2-D morphological autocorrelation transformation for melanoma diagnosis.Journal of Biomedical Optics 18, no. 12 (December 2013): 120502. https://doi.org/10.1117/1.jbo.18.12.120502.
Robles, Francisco E., et al. “Quantifying melanin spatial distribution using pump-probe microscopy and a 2-D morphological autocorrelation transformation for melanoma diagnosis.Journal of Biomedical Optics, vol. 18, no. 12, Dec. 2013, p. 120502. Epmc, doi:10.1117/1.jbo.18.12.120502.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

December 2013

Volume

18

Issue

12

Start / End Page

120502

Related Subject Headings

  • Skin Neoplasms
  • Skin
  • Optics
  • Microscopy
  • Melanoma
  • Melanins
  • Image Processing, Computer-Assisted
  • Humans
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering