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Repeated primary blast injury causes delayed recovery, but not additive disruption, in an in vitro blood-brain barrier model.

Publication ,  Journal Article
Hue, CD; Cao, S; Dale Bass, CR; Meaney, DF; Morrison, B
Published in: Journal of neurotrauma
May 2014

Recent studies have demonstrated increased susceptibility to breakdown of the cerebral vasculature associated with repetitive traumatic brain injury. We hypothesized that exposure to two consecutive blast injuries would result in exacerbated damage to an in vitro model of the blood-brain barrier (BBB) compared with exposure to a single blast of the same severity. Contrary to our hypothesis, however, repeated mild or moderate primary blast delivered with a 24 or 72 h interval between injuries did not significantly exacerbate reductions in transendothelial electrical resistance (TEER) across a brain endothelial monolayer compared with sister cultures receiving a single exposure of the same intensity. Permeability of the barrier to a range of different-sized solutes remained unaltered after single and repeated blast, supporting that the effects of repeated blast on BBB integrity were not additive. Single blast exposure significantly reduced immunostaining of ZO-1 and claudin-5 tight junction proteins, but subsequent exposure did not cause additional damage to tight junctions. Although repeated blast did not further reduce TEER, the second exposure delayed TEER recovery in BBB cultures. Similarly, recovery of hydraulic conductivity through the BBB was delayed by a second exposure. Extending the interinjury interval to 72 h, the effects of multiple injuries on the BBB were found to be independent given sufficient recovery time between consecutive exposures. Careful investigation of the effects of repeated blast on the BBB will help identify injury levels and a temporal window of vulnerability associated with BBB dysfunction, ultimately leading to improved strategies for protecting warfighters against repeated blast-induced disruption of the cerebral vasculature.

Duke Scholars

Published In

Journal of neurotrauma

DOI

EISSN

1557-9042

ISSN

0897-7151

Publication Date

May 2014

Volume

31

Issue

10

Start / End Page

951 / 960

Related Subject Headings

  • Tight Junctions
  • Neurology & Neurosurgery
  • Mice
  • In Vitro Techniques
  • Immunohistochemistry
  • Endothelial Cells
  • Electric Impedance
  • Cells, Cultured
  • Capillary Permeability
  • Brain Injuries
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hue, C. D., Cao, S., Dale Bass, C. R., Meaney, D. F., & Morrison, B. (2014). Repeated primary blast injury causes delayed recovery, but not additive disruption, in an in vitro blood-brain barrier model. Journal of Neurotrauma, 31(10), 951–960. https://doi.org/10.1089/neu.2013.3149
Hue, Christopher D., Siqi Cao, Cameron R. Dale Bass, David F. Meaney, and Barclay Morrison. “Repeated primary blast injury causes delayed recovery, but not additive disruption, in an in vitro blood-brain barrier model.Journal of Neurotrauma 31, no. 10 (May 2014): 951–60. https://doi.org/10.1089/neu.2013.3149.
Hue CD, Cao S, Dale Bass CR, Meaney DF, Morrison B. Repeated primary blast injury causes delayed recovery, but not additive disruption, in an in vitro blood-brain barrier model. Journal of neurotrauma. 2014 May;31(10):951–60.
Hue, Christopher D., et al. “Repeated primary blast injury causes delayed recovery, but not additive disruption, in an in vitro blood-brain barrier model.Journal of Neurotrauma, vol. 31, no. 10, May 2014, pp. 951–60. Epmc, doi:10.1089/neu.2013.3149.
Hue CD, Cao S, Dale Bass CR, Meaney DF, Morrison B. Repeated primary blast injury causes delayed recovery, but not additive disruption, in an in vitro blood-brain barrier model. Journal of neurotrauma. 2014 May;31(10):951–960.
Journal cover image

Published In

Journal of neurotrauma

DOI

EISSN

1557-9042

ISSN

0897-7151

Publication Date

May 2014

Volume

31

Issue

10

Start / End Page

951 / 960

Related Subject Headings

  • Tight Junctions
  • Neurology & Neurosurgery
  • Mice
  • In Vitro Techniques
  • Immunohistochemistry
  • Endothelial Cells
  • Electric Impedance
  • Cells, Cultured
  • Capillary Permeability
  • Brain Injuries