Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.
Publication
, Journal Article
Nguyen, H; Badie, N; McSpadden, L; Pedrotty, D; Bursac, N
Published in: Methods in molecular biology (Clifton, N.J.)
January 2014
Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes.
Duke Scholars
Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2014
Volume
1181
Start / End Page
249 / 262
Related Subject Headings
- Patch-Clamp Techniques
- Myocytes, Cardiac
- Humans
- Electrophysiological Phenomena
- Developmental Biology
- Cell Communication
- Action Potentials
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Nguyen, H., Badie, N., McSpadden, L., Pedrotty, D., & Bursac, N. (2014). Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs. Methods in Molecular Biology (Clifton, N.J.), 1181, 249–262. https://doi.org/10.1007/978-1-4939-1047-2_21
Nguyen, Hung, Nima Badie, Luke McSpadden, Dawn Pedrotty, and Nenad Bursac. “Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.” Methods in Molecular Biology (Clifton, N.J.) 1181 (January 2014): 249–62. https://doi.org/10.1007/978-1-4939-1047-2_21.
Nguyen H, Badie N, McSpadden L, Pedrotty D, Bursac N. Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs. Methods in molecular biology (Clifton, NJ). 2014 Jan;1181:249–62.
Nguyen, Hung, et al. “Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.” Methods in Molecular Biology (Clifton, N.J.), vol. 1181, Jan. 2014, pp. 249–62. Epmc, doi:10.1007/978-1-4939-1047-2_21.
Nguyen H, Badie N, McSpadden L, Pedrotty D, Bursac N. Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs. Methods in molecular biology (Clifton, NJ). 2014 Jan;1181:249–262.
Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2014
Volume
1181
Start / End Page
249 / 262
Related Subject Headings
- Patch-Clamp Techniques
- Myocytes, Cardiac
- Humans
- Electrophysiological Phenomena
- Developmental Biology
- Cell Communication
- Action Potentials
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology