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Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels.

Publication ,  Journal Article
Yang, H; Hu, L; Shi, J; Delaloye, K; Horrigan, FT; Cui, J
Published in: Proc Natl Acad Sci U S A
November 13, 2007

The voltage-sensor domain (VSD) of voltage-dependent ion channels and enzymes is critical for cellular responses to membrane potential. The VSD can also be regulated by interaction with intracellular proteins and ligands, but how this occurs is poorly understood. Here, we show that the VSD of the BK-type K(+) channel is regulated by a state-dependent interaction with its own tethered cytosolic domain that depends on both intracellular Mg(2+) and the open state of the channel pore. Mg(2+) bound to the cytosolic RCK1 domain enhances VSD activation by electrostatic interaction with Arg-213 in transmembrane segment S4. Our results demonstrate that a cytosolic domain can come close enough to the VSD to regulate its activity electrostatically, thereby elucidating a mechanism of Mg(2+)-dependent activation in BK channels and suggesting a general pathway by which intracellular factors can modulate the function of voltage-dependent proteins.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

November 13, 2007

Volume

104

Issue

46

Start / End Page

18270 / 18275

Location

United States

Related Subject Headings

  • Static Electricity
  • Potassium Channels
  • Mice
  • Magnesium
  • Large-Conductance Calcium-Activated Potassium Channels
  • Cytosol
  • Animals
 

Citation

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Yang, H., Hu, L., Shi, J., Delaloye, K., Horrigan, F. T., & Cui, J. (2007). Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels. Proc Natl Acad Sci U S A, 104(46), 18270–18275. https://doi.org/10.1073/pnas.0705873104
Yang, Huanghe, Lei Hu, Jingyi Shi, Kelli Delaloye, Frank T. Horrigan, and Jianmin Cui. “Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels.Proc Natl Acad Sci U S A 104, no. 46 (November 13, 2007): 18270–75. https://doi.org/10.1073/pnas.0705873104.
Yang H, Hu L, Shi J, Delaloye K, Horrigan FT, Cui J. Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18270–5.
Yang, Huanghe, et al. “Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels.Proc Natl Acad Sci U S A, vol. 104, no. 46, Nov. 2007, pp. 18270–75. Pubmed, doi:10.1073/pnas.0705873104.
Yang H, Hu L, Shi J, Delaloye K, Horrigan FT, Cui J. Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18270–18275.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

November 13, 2007

Volume

104

Issue

46

Start / End Page

18270 / 18275

Location

United States

Related Subject Headings

  • Static Electricity
  • Potassium Channels
  • Mice
  • Magnesium
  • Large-Conductance Calcium-Activated Potassium Channels
  • Cytosol
  • Animals